1.25139
Fractogel® EMD SO3 ⁻ (M) Resin
RoboColumn Fractogel® SO3 (M), volume 0.6 mL, prepacked column
Synonym(s):
RoboColumn Fractogel® SO3 (M), 0.6 ml
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About This Item
UNSPSC Code:
41115711
ligand
(Sulfoisobutyl)
Quality Level
material
polypropylene housing
product line
Fractogel®
form
prepacked column
parameter
≤8 bar pressure
~200 cm/hr max. flow rate
column I.D. × L
5 mm × 30 mm
volume
0.6 mL
matrix active group
Methacrylate
mean particle size
40-90 μm
capacity
~130 mL, resin binding capacity
application(s)
mAb
recombinant protein
vaccine development
separation technique
cation exchange
storage temp.
15-25°C
General description
5 x 30 mm
Features and Benefits
RoboColumn Fractogel® SO3 (M), 0.6 ml enables:
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- Increased binding strength at low pH
- High aggregate removal efficiency combined with high dynamic binding capacity
- Lower elution volumes for the highest purity levels
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
Legal Information
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
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