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Sigma-Aldrich

Anti-α-Actin Mouse mAb (1A4)

liquid, clone 1A4, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody product type

primary antibodies

clone

1A4, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

baboon, rabbit, human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ACTA1(58)

General description

Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
  • Antibody Target Gene Symbol: ACTA2
  • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
  • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
  • Hu Entrez ID: 59 (Related Antibodies: 113200)
  • Rat Entrez ID: 81633
    • This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

    Immunogen

    Human Smooth Muscle
    a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

    Application

    Frozen Sections (1:50-1:75)

    Immunoblotting (see comments)

    Paraffin Sections (1:50-1:75)

    Packaging

    Please refer to vial label for lot-specific concentration.

    Warning

    Toxicity: Standard Handling (A)

    Physical form

    In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

    Analysis Note

    Negative Control
    Fibroblasts, striated muscle, or myocardium
    Positive Control
    Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary

    Other Notes

    Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
    Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
    Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
    Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    Storage Class Code

    12 - Non Combustible Liquids

    WGK

    WGK 2

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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