05-1471

Anti-Rictor Antibody, clone 9F1.2

clone 9F1.2, from mouse

• Synonym(s): Rictor, AVO3, rapamycin-insensitive companion of mTOR, mAVO3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9F1.2, monoclonal
• species reactivity: human, mouse, rat
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_689969.2
• UniProt accession no.: Q6R327
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RICTOR(253260)

Description

General description

Rictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/GL. mTORC2 is activated by growth factors and other mitogenic signals.

Specificity

This antibody recognizes Rictor.

Immunogen

Recombinant protein corresponding to human Rictor.

Application

Anti-Rictor Antibody, clone 9F1.2 detects level of Rictor & has been published & validated for use in WB & IP.

Immunoprecipitation:
Representative lot data.
1 ug/mL dilution of this antibody immunoprecipitated Rictor from 100 mg of HEK293 lysate.

Quality

Evaluated by Western Blotting on 10 mg of HEK293 cell lysate.

Target description

190 kDa Observed

Physical form

Format: Purified

Purified immunoglobulin in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Analysis Note

Control
HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Rictor on 10 µg of HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable