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Sigma-Aldrich

Ammonium-15N chloride

≥98 atom % 15N, ≥99% (CP)

Synonym(s):

15N Labeled ammonium chloride

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About This Item

Linear Formula:
15NH4Cl
CAS Number:
Molecular Weight:
54.48
MDL number:
UNSPSC Code:
12142200
PubChem Substance ID:
NACRES:
NA.12

isotopic purity

≥98 atom % 15N

Assay

≥99% (CP)

technique(s)

bio NMR: suitable
protein expression: suitable

mp

340 °C (subl.) (lit.)

mass shift

M+1

SMILES string

[15NH4+].[Cl-]

InChI

1S/ClH.H3N/h1H;1H3/i;1+1

InChI key

NLXLAEXVIDQMFP-IEOVAKBOSA-N

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Application

Ammonium-15N chloride has been used:
  • as a medium supplement for the incorporation of 15N in sporulation protein, CsfB, for NMR spectroscopy
  • in assaying nitrogen loss in marine sediment
  • for the incorporation of 15N in apolipoprotein A-I for mass spectrum analysis

Biochem/physiol Actions

Ammonium chloride is a component of the nitrogen cycle. Excess of ammonium chloride depletes ATP in astrocytes. Ammonium chloride inhibits the acidification in endosome-lysosome system. It is a hemolysing agent used in the lysis of erythrocytes. Ammonium chloride administration promotes creatinine and urea clearance in rat.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Emergence of resistance to antibacterial agents: the role of quaternary ammonium compounds?a critical review
Buffet-Bataillon S, et al.
International Journal of Antimicrobial Agents, 39(5), 381-389 (2012)
Anaerobic ammonium oxidation linked to sulfate and ferric iron reduction fuels nitrogen loss in marine sediments
Rios-Del T, et al.
Biodegradation, 29(5), 429-442 (2018)
Characterization of homodimer interfaces with cross-linking mass spectrometry and isotopically labeled proteins
Lima DB, et al.
Nature Protocols, 13(3), 431-431 (2018)
Journal of the Chemical Society. Chemical Communications, 62-62 (1982)
Thomas Wiegand et al.
Journal of biomolecular NMR, 65(2), 79-86 (2016-06-01)
The use of protein building blocks for the structure determination of multidomain proteins and protein-protein complexes, also known as the "divide and conquer" approach, is an important strategy for obtaining protein structures. Atomic-resolution X-ray or NMR data of the individual

Articles

Solid-state NMR on Larger Biomolecules; Sigma-Aldrich.com

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