APP modulates KCC2 expression and function in hippocampal GABAergic inhibition.

APP modulates KCC2 expression and function in hippocampal GABAergic inhibition.

eLife (2017-01-06)

Ming Chen, Jinzhao Wang, Jinxiang Jiang, Xingzhi Zheng, Nicholas J Justice, Kun Wang, Xiangqian Ran, Yi Li, Qingwei Huo, Jiajia Zhang, Hongmei Li, Nannan Lu, Ying Wang, Hui Zheng, Cheng Long, Li Yang

PMID28054918

RÉSUMÉ

Amyloid precursor protein (APP) is enriched at the synapse, but its synaptic function is still poorly understood. We previously showed that GABAergic short-term plasticity is impaired in

MATÉRIAUX

Référence du produit

Marque

Description du produit

C1768

Sigma-Aldrich

Cytosine β-D-arabinofuranoside, crystalline, ≥90% (HPLC)

T6557

Sigma-Aldrich

Anti-γ-Tubulin antibody, Mouse monoclonal, clone GTU-88, ascites fluid

07-432

Sigma-Aldrich

Anticorps anti-cotransporteur K+/Cl- (KCC2), Upstate^®, from rabbit

06-868

Sigma-Aldrich

Anticorps anti-récepteur α1 GABAA, Upstate^®, from rabbit

16-452

Sigma-Aldrich

4G10^® Platinum, Anti-Phosphotyrosine Antibody, Biotin Conjugate, clone 4G10®, Upstate^®, from mouse