16-452
4G10® Platinum, Anti-Phosphotyrosine Antibody, Biotin Conjugate
clone 4G10®, Upstate®, from mouse
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
biotin conjugate
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
4G10®, monoclonal
Réactivité de l'espèce (prédite par homologie)
all
Fabricant/nom de marque
Upstate®
Technique(s)
western blot: suitable
Isotype
IgG2bκ
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PID1(55022)
Description générale
The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. Now Millipore has made the best even better with 4G10 Platinum. We pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20, to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone is capable.
Immunogène
The immunogen for 4G10 was phospho-tyramine coupled to KLH, while the immunogen for PY20 was phosphotyrosine conjugated to a carrier protein.
Application
4G10 Platinum, Anti-Phosphotyrosine, Biotin Conjugate detects levels of Phosphotyrosine proteins & has been published & validated for use in IP.
Qualité
Western Blot Analysis:
A dilution of 1:1000-2000 of this antibody detected tyrosine-phosphorylated proteins in a modified RIPA lysate from human HEK293 cells treated with 100 nM insulin.
A dilution of 1:1000-2000 of this antibody detected tyrosine-phosphorylated proteins in a modified RIPA lysate from human HEK293 cells treated with 100 nM insulin.
Description de la cible
Varies
Forme physique
100 μL of a proprietary mixture of biotin conjugated, protein G purified mouse monoclonals 4G10 (IgG2bκ) and PY20 (IgG2b) in PBS, pH 7.4 with 0.05% sodium azide.
Stockage et stabilité
Stable for 6 months at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
NOTE: DO NOT FREEZE.
NOTE: DO NOT FREEZE.
Informations légales
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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International journal of biological sciences, 9(10), 1099-1107 (2013-12-18)
A novel assay was developed to measure ratio of p-FMS (phospho FMS) to FMS using the Meso Scale Discovery(®) (MSD) technology and compared to the routinely used, IP-Western based approach. The existing IP-Western assay used lysed PBMCs (Peripheral Blood Mononuclear
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The Journal of clinical investigation, 117(6), 1527-1537 (2007-05-19)
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