- The activation of the atypical PKC zeta in light-induced retinal degeneration and its involvement in L-DNase II control.
The activation of the atypical PKC zeta in light-induced retinal degeneration and its involvement in L-DNase II control.
Journal of cellular and molecular medicine (2015-03-18)
Imene Jaadane, Sabine Chahory, Chloé Leprêtre, Boubaker Omri, Laurent Jonet, Francine Behar-Cohen, Patricia Crisanti, Alicia Torriglia
PMID25781645
RÉSUMÉ
Light-induced retinal degeneration is characterized by photoreceptor cell death. Many studies showed that photoreceptor demise is caspase-independent. In our laboratory we showed that leucocyte elastase inhibitor/LEI-derived DNase II (LEI/L-DNase II), a caspase-independent apoptotic pathway, is responsible for photoreceptor death. In this work, we investigated the activation of a pro-survival kinase, the protein kinase C (PKC) zeta. We show that light exposure induced PKC zeta activation. PKC zeta interacts with LEI/L-DNase II and controls its DNase activity by impairing its nuclear translocation. These results highlight the role of PKC zeta in retinal physiology and show that this kinase can control caspase-independent pathways.
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Bleu de thiazol (thiazolyl blue tetrazolium bromide), powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
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Cycloheximide solution, Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture