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575664-U

Supelco

Supel-Select SCX SPE 96-well Plate

bed wt. 30 mg/well, pk of 1

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About This Item

Code UNSPSC :
41104018
Nomenclature NACRES :
NB.21

Matériaux

PE frit (20 μm)
polypropylene hardware

Qualité

MS Friendly

Composition

bed wt., 30 mg/well

Conditionnement

pk of 1

Technique(s)

solid phase extraction (SPE): suitable

Superficie

160-420 m2/g

Matrice

Sulfonic acid functionalized hydrophilic modified styrene polymer particle platform

Groupe de la matrice active

sulfonic acid phase

Taille des particules

50-70 μm

Dimension de pores

80-200 Å pore size

Application(s)

food and beverages

Technique de séparation

ion exchange

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Description générale

Retention Mechanism: Ion-Exchange
Sample Matrix Compatibility: Aqueous solutions (biological fluids, water)

  • Sulfonic acid functionalized hydrophilic modified styrene polymer
  • Developed for the extraction of a highly broad range of compounds from aqueous samples
  • Retention predominately based on ion-exchange; however, because the phase is hydrophilic modified, it is amenable to polar compounds as well.

Caractéristiques et avantages

  • Excellent sample prep performance at a lower price
  • Amenable to generic methodology - save time, money, and headache during method development
  • Greater capacity allows for smaller bed weights = smaller elution volumes = time savings in sample processing
  • Resistance to overdrying allows for more robust methdology
  • Low UV and MS extractables for lower background and greater sensitivity
  • Stringent production and QC guidelines offer greater lot-to-lot, tube-to-tube, and well-to-well consistency for improved accuracy and precision

Informations légales

Supel is a trademark of Sigma-Aldrich Co. LLC

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°C)

Not applicable


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Articles

Shown here is the chiral separation of amphetamine enantiomers under MS-compatible conditions on Astec CHIROBIOTIC® V2 after extraction from urine using Supel™-Select SCX SPE 96-well plates. The highest grade mobile phase solvents and additives were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

SPE retention mechanism in this case is based on the electrostatic attraction of charged functional groups of the analyte(s) to oppositely charged functional groups on the sorbent.

Protocoles

In this study, optimized methods are presented for sample preparation and chiral chromatography for the LC/MS analysis of amphetamine and methamphetamine enantiomers in urine.

Optimized sample prep and chiral chromatography methods for the LC/MS analysis of these drug enantiomers in urine

LC/MS Analysis of Methamphetamine Enantiomers on Astec® CHIROBIOTIC® V2 in Urine after SPE using Supel™-Select SCX

Retention occurs through polar interaction between the sorbent and analytes. Typical sample matrices that can be employed in normal-phase SPE include hydrocarbon or fatty oils diluted in a solvent like hexane, isooctane, chlorinated solvent, THF, diethyl ether, or ethyl acetate.

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