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50603-U

Supelco

Ascentis® Express F5, 5 μm HPLC Column

5 μm particle size, L × I.D. 2 cm × 2.1 mm

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About This Item

Code UNSPSC :
41115700
eCl@ss :
32110501
Nomenclature NACRES :
SB.52

Matériaux

stainless steel column

Niveau de qualité

100

Agence

suitable for USP L43

Gamme de produits

Ascentis®

Caractéristiques

endcapped

Fabricant/nom de marque

Ascentis®

Conditionnement

1 ea of

Paramètres

60 °C temp. range
600 bar max. pressure (9000 psi)

Technique(s)

HPLC: suitable
LC/MS: suitable

L × D.I.

2 cm × 2.1 mm

Superficie

90 m2/g

Impuretés

<5 ppm metals

Matrice

Fused-Core particle platform
superficially porous particle

Groupe de la matrice active

PFP (pentafluorophenyl) phase

Taille des particules

5 μm

Dimension de pores

90 Å pore size

pH de fonctionnement

2-8

Application(s)

food and beverages

Technique de séparation

reversed phase

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Catégories apparentées

Description générale

Ascentis® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

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Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
USBU001262
USBU001261
USBU001260
USBU001259
USBU001258

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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Imran Ali et al.
Journal of separation science, 35(23), 3235-3249 (2012-11-28)
New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra-fast HPLC separations for a variety of compounds with moderate
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 120, 112-119 (2016-01-01)
A new high-performance liquid chromatography method using fused-core column for fast separation of resveratrol and polydatin has been developed and used for quality control of nutraceuticals with resveratrol and polydatin content. Retention characteristics (log k) were studied under different conditions
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