ZRB2431

Anti-Interleukin-2 Antibody, clone 1F22-L2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Aldesleukin, T-cell growth factor (TCGF)
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1F22-L2, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 18 kDa; observed mol wt ~16 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey
• Conditionnement: antibody small pack of 25
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; flow cytometry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: Internal
• Numéro d'accès Protein ID: NP_000577
• Numéro d'accès UniProt: P60568
• Autre catégorie plus écologique: Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... IL2(3558)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1F22-L2 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Interleukin-2. It targets an epitope within 17 amino acids from the internal part of the protein.

Immunogène

KLH-conjugated linear peptide corresponding to 17 amino acids from the internal part of human Interleukin-2.

Application

Quality Control Testing

Evaluated by Western Blotting using recombinant human Interleukin-2.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant human Interleukin-2.

Tested Applications

Affinity Binding Assay: The binding affinity of this antibody to Interleukin-2 peptide was at least one thousand-fold (1,000X) higher than that obtained with the non-specific control peptide.

Flow Cytometry Analysis: 1 µg from a representative lot detected Interleukin-2 in Jurkat cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Interleukin-2 (UniProt: P60568; also known as T-cell growth factor (TCGF), Aldesleukin) is encoded by the IL2 gene (Gene ID: 3558) in human. Interleukin-2 (IL-2) Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells. IL-2 plays a crucial role in regulating the growth, activation, and differentiation of various immune cells. IL-2 consists of a four-helix bundle and belongs to the four-helix cytokine family. It interacts with specific receptors on the surface of target cells, initiating signaling pathways that lead to immune cell activation and proliferation. IL-2 supports the growth and survival of T cells. It acts as a growth factor for T cells and stimulates their differentiation into effector cells, such as cytotoxic T cells and regulatory T cells. IL-2 also enhances the production of other cytokines and promotes the development of long-lasting immune responses. IL-2 is produced by various immune cells, including activated T cells and natural killer (NK) cells. Its expression is tightly regulated and can be induced by immune activation and antigen stimulation. IL-2 expression levels can vary depending on the immune cell type, activation status, and surrounding microenvironment. IL-2 plays a critical role in numerous immune-related diseases and conditions. For example, deficiencies or dysregulation of IL-2 signaling can lead to immune dysfunction and result in autoimmune disorders, immunodeficiency diseases, and certain types of cancers. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable