ZRB2276

Anti-CUX1 Antibody, clone 1J18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : CCAAT displacement protein, CDP, Homeobox protein Cux-1, Homeobox protein cut-like 1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1J18, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 164.19 kDa; observed mol wt ~230 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: C-terminal half
• Numéro d'accès Protein ID: NP_853530
• Numéro d'accès UniProt: P39880
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... CUX1(1523)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1J18 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Homeobox protein cut-like 1 (CUX1). It targets an epitope within 21 amino acids from the C-terminal half.

Immunogène

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal half of human Homeobox protein cut-like 1 (CUX1).

Application

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell nuclear extract.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CUX1 in HEK293 cell nuclear extract.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CUX1 in Human cerebellum tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound CUX1 peptide with a KD of 6.7 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CUX1 in K562 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Homeobox protein cut-like 1 (UniProt: P39880; also known as CCAAT displacement protein, CDP, Homeobox protein cux-1, CCAAT displacement protein, CDP, Homeobox protein cux-1) is encoded by the CUX1 (also known as CUTL1) gene (Gene ID: 1523) in human. Homebox protein CUX1 is member of the CUT Homebox family that has a broad role in mammalian development. It is a transcription factor that is involved in the control of neuronal differentiation in the brain. It regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III. It has a conserved coiled-coil region at the N terminus (aa 56-407), three internal 60-amino-acid repeats (Cut repeats; 542-629; 934-1021; and 1117-1204), and a divergent homeodomain located near the C terminus (aa 1244-1303). CUX1 serves as a repressor of developmentally regulated gene expression and acts by preventing binding of positively-acting CCAAT factors to promoters. It is also a component of NF-muNR repressor, which binds to the matrix attachment regions (MARs) (5′ and 3′) of the immunoglobulin heavy chain enhancer. CUX1 is shown to repress T-cell receptor β enhancer function by binding to the matrix attachment region beta (MARβ), which is an ATC-rich DNA sequence located upstream of the TCR β enhancer. Its cleaved product, CDP/Cux p110, is reported to play a role in cell cycle progression, in particular at the G1/S transition. While CUX1 only transiently binds to DNA and carries the CCAAT-displacement activity, CDP/Cux p110 makes a stable interaction with DNA and stimulates expression of genes such as POLA1. Eleven different isoforms of CUX1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Goulet, B., et al. (2004). Mol. Cell. 14(2); 207-219; Ellis, T., et al. (2001). Genes Dev. 15 (17); 2307-2319).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable