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ZRB1982

Sigma-Aldrich

Anti-IL-6 Antibody, clone 3B9 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonyme(s) :

Anti-Anti-BSF-2, Anti-Anti-BSF2, Anti-Anti-CDF, Anti-Anti-HGF, Anti-Anti-HSF, Anti-Anti-IFN-beta-2, Anti-Anti-IFNB2, Anti-Anti-IL-6

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

3B9, recombinant monoclonal

Description

3B9 Clone

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Poids mol.

calculated mol wt 23.72 kDa
observed mol wt ~21 kDa

Espèces réactives

human

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Technique(s)

ELISA: suitable
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable

Isotype

IgG

Séquence de l'épitope

Unknown

Numéro d'accès Protein ID

Numéro d'accès UniProt

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 3B9 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human IL-6.

Immunogène

His-tagged recombinant full length human IL-6 (without the signal peptide).

Application

Quality Control Testing

Evaluated by Western Blotting in human recombinant IL-6.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected human recombinant IL-6.

Tested applications

Affinity Binding Assay Analysis: A representative lot of this antibody bound IL-6 with a KD of 1.0 x 10^-12 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IL-6 in K562 cell line.

ELISA Analysis: A representative lot of this antibody detected human recombinant IL-6 in ELISA application.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-6, clone 3B9 ZooMAb®, Cat. No. ZRB1982, is a recombinant Rabbit monoclonal antibody that specifically targetsIL-6 and is tested for use in Affinity Binding Assay, ELISA, Immunocytochemistry, and Western Blotting.

Description de la cible

Interleukin-6 (UniProt: P05231; also known as IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2) is encoded by the IL6 (also known as IFNB2) gene (Gene ID: 3569) in human. IL-6 is a proinflammatory cytokine that is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off to generate the mature form. It is produced by monocytes, endothelial cells, and adipose tissue. IL-6 is involved in a variety of biological functions and is a potent inducer of acute phase response. In the innate immune response. It is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. However, in the adaptive immune response, it is required for the differentiation of B cells into immunoglobulin-secreting cells. It also plays a major role in the differentiation of CD4+ T cell subsets and in proliferation of myeloma cells and the survival of plasma blast cells. It drives naive CD4+ T cells to the Th17 lineage. IL-6 is reported to discharge into the bloodstream after muscle contraction and increase lipolysis and improves insulin resistance. Rapid production of IL-6 contributes to host defense during infection and tissue injury, but excessive IL-6 synthesis contributes to disease pathology. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wolsk, E., et al. (2010). Am. J. Physiol. Endocrinol. Metab. 299(5); E832-840; Tanaka, T., et al. (2014). Cold Spring Harb. Perspect. Biol. 4(6); a016295).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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