ZRB1532

Anti-SMARCA2 Antibody, clone 3E14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Probable global transcription activator SNF2L2;ATP-dependent helicase SMARCA2;BRG1-associated factor 190B;BAF190B;Protein brahma homolog;hBRM;SNF2-alpha;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 3E14, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 181.28 kDa; observed mol wt ~180 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: C-terminal
• Numéro d'accès Protein ID: NP_001276325
• Numéro d'accès UniProt: P51531
• Autre catégorie plus écologique: Aligned,
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SMARCA2(6595)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 3E14 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects SMARCA2. It targets an epitope within 24 amino acids from the C-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human SMARCA2.

Application

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected SMARCA2 in MCF-7 cell lysate.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected SMARCA2 in human testis tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound SMARCA2 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Western Blotting Analysis): A 1:1,000 dilution from a representative lot detected SMARCA2 in lysate from THP-1 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SMARCA2 in U2OS cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected SMARCA2 in MCF-7 cell lysate.

Description de la cible

Probable global transcription activator SNF2L2 (UniProt: P51531; also known as ATP-dependent helicase SMARCA2, BRG1-associated factor 190B, BAF190B, Protein brahma homolog, hBRM, SNF2-alpha, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2) is encoded by the SMARCA2 (also known as BAF190B, BRM, SNF2A, SNF2L2) gene (Gene ID: 6595) in human. SMARCA2 is a member of the SNF2/RAD54 helicase family and belongs to the neural progenitors-specific chromatin remodeling complex and the neuron-specific chromatin remodeling complex (nBAF complex). It is involved in transcriptional activation and repression of select genes by chromatin remodeling. During differentiation from embryonic stem cells to neural progenitor cells, SMARCA4 expression gradually declines and SMARCA2 is upregulated and continues to be highly expressed in neurons. SMARCA2 also has a tumor suppressor function, and its expression is associated with good prognosis. Its high expression is associated with benign differentiated tumors. It is found to be silenced in a number of cancer cell lines and primary tumors. Heterozygous missense mutations in the SMARCA2 gene have been linked to Nicolaides-Baraitser syndrome (NCBRS) that is characterized by severe mental retardation. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gao, F., et al. (2019). Mol. Cell. 75; 891-904; Guerrero-Martinez, JA., and Reyes, JC. (2018). Sci. Rep. 8; Article 2043).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable