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ZRB1454

Sigma-Aldrich

Anti-TIGIT Antibody, clone 1D20 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonyme(s) :

T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit (recombinant)

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

1D20, monoclonal
recombinant monoclonal

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Poids mol.

calculated mol wt 26.32 kDa
observed mol wt 40 kDa

Espèces réactives

human, mouse, rat

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Technique(s)

affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotype

IgG

Numéro d'accès UniProt

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TIGIT(201633)

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 1D20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human T-cell immunoreceptor with Ig and ITIM domains (TIGIT). It targets an epitope within 16 amino acids from the extracellular domain.

Immunogène

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal, extracellular domain of human T-cell immunoreceptor with Ig and ITIM domains (TIGIT).

Application

Anti-TIGIT, clone 1D20 ZooMAb, Cat. No. ZRB1454, is a recombinant Rabbit monoclonal antibody that specifically targets TIGIT and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TIGIT in Rat Spleen, Mouse Thymus, and Human Spleen tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TIGIT in Jurkat and K562 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected TIGIT in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound TIGIT peptide with a KD of value of 3.6 x 10-9 in an affinity binding assay.

Flow Cytometry Analysis: 1 µg of a representative lot of this antibody detected TIGIT in one million Raw264.7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Description de la cible

T-cell immunoreceptor with Ig and ITIM domains (UniProt: Q495A1; also known as V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3, TIGIT) is encoded by the TIGIT (also known as VSIG9, VSTM3) gene (Gene ID: 201633) in human. TIGIT is a single-pass type I membrane protein that binds with high affinity to the poliovirus receptor (PVR) forming a heterotetrameric assembly of two TIGIT and two PVR molecules. This causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. It is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells. It is also expressed on many cell types important in cancer, and its mechanism of action may depend on the cell type studied. TIGIT also serves as a functional ligand and induces dendritic cells to acquire a tolerogenic phenotype via ligation of PVR. TIGIT -/- mice, when challenged, develop severe experimental autoimmune encephalomyelitis compared to wild-type mice. TIGIT is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-141), a transmembrane domain (aa 142-162), and a cytoplasmic domain (aa 163-244). The cytoplasmic domain contains an immunoreceptor tyrosine-based inhibitor motif (ITIM; aa 229-234), which is involved in the modulation of cellular responses. The IgV domain (aa 22-124) of TIGIT possesses distinctive (V/I)(S/T)Q, AX6G, and T(F/Y)PX1G sub-motifs that mediate a ′lock and key′ trans-interaction with cis-homodimers of PVR. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Manieri, NA., et al. (2017). Trends Immunol. 38(1); 20-28).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1


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