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Key Documents

ZRB1312

Sigma-Aldrich

Anti-β-Actin Antibody, clone 6L12, ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonyme(s) :

Actin cytoplasmic 1

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit (recombinant)

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

6L12, monoclonal
recombinant monoclonal

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

Poids mol.

calculated mol wt 41.74 kDa
observed mol wt ~42 kDa

Espèces réactives

rat, mouse, monkey, human

Réactivité de l'espèce (prédite par homologie)

bovine, porcine

Conditionnement

antibody small pack of 25 μL

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Validation améliorée

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotype

IgG

Numéro d'accès UniProt

Autre catégorie plus écologique

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ACTB(60)

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 6L12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects beta-Actin in multiple species. It targets an epitope within 16 amino acids from the N-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human beta-Actin.

Application

Anti-beta-Actin, clone 6L12 ZooMAb, Cat. No. ZRB1312, is a recombinant rabbit monoclonal antibody that specifically detects beta-Actin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected -Actin in C2C12, C6, and COS-1 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected -Actin in human uterus and human stomach tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected -Actin in C2C12 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Actin, cytoplasmic 1 (UniProt: P60709; also known as beta-Actin) is encoded by the ACTB gene (Gene ID: 60) in human. Actins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Beta- and gamma-actins are the major structural components of the actin cytoskeleton in non-muscle cells. Beta-actin plays a predominant role in cell migration and its enrichment has also been observed at the leading edge of migrating cells, whereas gamma-actin appears to be more uniformly distributed throughout the cell. In addition, beta-actin can also undergo N-terminal arginylation that regulates lamella formation in motile cells. Beta-actin has also been consistently utilized as a loading control in Western blotting applications due to the fact that its expression is fairly consistent, although its levels can somewhat differ in different tissues and its expression can be affected by certain pathological conditions. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Leilei Lin et al.
Journal of translational medicine, 19(1), 123-123 (2021-03-25)
As a common haematological malignancy, acute myeloid leukaemia (AML), particularly with extramedullary infiltration (EMI), often results in a high mortality rate and poor prognosis. Circular RNAs (circRNAs) regulate biological and pathogenic processes, suggesting a potential role in AML. We have
Lucy G Thorne et al.
The EMBO journal, 40(15), e107826-e107826 (2021-06-09)
SARS-CoV-2 infection causes broad-spectrum immunopathological disease, exacerbated by inflammatory co-morbidities. A better understanding of mechanisms underpinning virus-associated inflammation is required to develop effective therapeutics. Here, we discover that SARS-CoV-2 replicates rapidly in lung epithelial cells despite triggering a robust innate
Bo Lu et al.
Oncology letters, 21(4), 306-306 (2021-03-19)
Multiple myeloma (MM) is the second most common hematopoietic malignancy and remains an incurable disease. Thus, novel drugs and therapeutic methods are required for patients with MM. The present study aimed to investigate the effect of sirtuin 1 (SIRT1) inhibitor
Xi Wang et al.
Oncology reports, 44(3), 1025-1036 (2020-07-25)
Emerging evidence has demonstrated that abnormally expressed long non‑coding (lnc) RNAs contribute to drug resistance in various types of malignancy. LncRNA antisense non‑coding RNA in the inhibitor of cyclin‑dependent kinase 4 locus (ANRIL) exerts oncogenic activity and acts as a
Yu Huang et al.
Journal of the American Heart Association, 10(21), e021895-e021895 (2021-10-30)
Background Exploring potential therapeutic target is of great significance for myocardial infarction (MI) and post-MI heart failure. Transcription factor Yin-Yang 1 (YY1) is an essential regulator of apoptosis and angiogenesis, but its role in MI is unclear. Methods and Results

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