ZRB06711

Anti-FADD Antibody, clone 11N7, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit (recombinant)
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 11N7, recombinant monoclonal; monoclonal
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 23.28 kDa; observed mol wt ~26 kDa
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): gene knockdown: suitable using 1:250; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100; western blot: suitable using 1:1,000
• Isotype: IgG
• Numéro d'accès UniProt: Q13158
• Autre catégorie plus écologique: , Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FADD;MORT1;GIG3(8772)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 11N7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human FAS-associated death domain protein (FADD).

Immunogène

His-tagged full-length recombinant human FAS-associated death domain protein (FADD).

Application

Anti-FADD, clone 11N7, ZooMAb, Cat. No. Cat. No. ZRB06711, is a recombinant Rabbit Monoclonal antibody that detects human FAS-associated death domain and has been tested for use in Immunohistochemistry (Paraffin), RNAi/siRNA/Gene Knockdown, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected FADD in MCF7 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FADD in human kidney tissue sections.

RNAi/siRNA/Gene Knockdown Analysis: A 1:250 dilution from a representative lot detected FADD lysates from Wild type HeLa cells, but not in FADD knockout HeLa cells.

Description de la cible

FAS-associated death domain protein (UniProt: Q13158; also known as FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD) is encoded by the FADD (also known as MORT1, GIG3) gene (Gene ID: 8772) in human. FADD is an apoptotic adaptor molecule that bridges death receptor signaling to the caspase cascade. It is expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. FADD contains a death domain at its C-terminus and a death effector domain (DED) at its N-terminal. The death domain is responsible for FADD binding to the activated trimerized Fas receptor. FADD recruits procaspase-8 through its N-terminal DED, which is then activated following the cleavage of pro domain. The death inducing signaling complex (DISC) formed by Fas receptor, FADD, and caspase-8 is essential in triggering apoptotic events in cells. Human FADD is phosphorylated mainly at Ser194. Defects in FADD gene have been linked to recurrent infections, hepatic dysfunction, and cardiovascular malformations. These subjects display high circulating levels of CD4(-) and CD8(-) cells and elevated levels of IL-10. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2