W4769
Monoclonal Anti-WIPI-1 antibody produced in mouse
~1.5 mg/mL, clone WIPI1-4, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
Anti-ATG18, Anti-WIPI-1α, Anti-WIPI49
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About This Item
Code UNSPSC :
12352203
Conjugué:
unconjugated
application:
WB
Clone:
WIPI1-4, monoclonal
Espèces réactives:
human, mouse, bovine, rat
citations:
2
Technique(s):
western blot: 2-4 μg/mL using whole extract of G361 cells
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
WIPI1-4, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~49 kDa
Espèces réactives
human, mouse, bovine, rat
Concentration
~1.5 mg/mL
Technique(s)
western blot: 2-4 μg/mL using whole extract of G361 cells
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... WIPI1(55062)
mouse ... Wipi1(52639)
rat ... Wipi1(303630)
Description générale
Monoclonal Anti-WIPI-1 (mouse IgG1 isotype) is derived from the hybridoma WIPI1-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human WIPI-1, conjugated to KLH. WIPI-1 (WD repeat domain phosphoinositide-interacting protein 1) the mammalian orthologue of Atg18 in S. cerevisiae and A. thaliana, is a member of the WIPI subfamily of WD-repeat (Trp-Asp) proteins. WIPI-1 has a 7-bladed propeller structure and contains a conserved motif for interaction with phospholipids. WIPI-1 is expressed in normal human tissues with high levels in skeletal muscle, heart and testis.
Application
Monoclonal Anti-WIPI-1 antibody has been used in immunoblotting, immunoprecipitation.
Actions biochimiques/physiologiques
WIPI-1 (WD repeat domain phosphoinositide-interacting protein 1) plays an important role in signal transduction, transcription regulation and apoptosis. Endogenous WIPI-1 partially colocalizes with the autophagosomal marker LC3 and induction of autophagy leads to the accumulation of WIPI-1 in large vesicular and cup-shaped structures that are characteristic for autophagy-linked proteins. WIPI-1 is aberrantly expressed in human cancer and is linked to starvation induced autophagy.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Noboru Mizushima et al.
Cell structure and function, 27(6), 421-429 (2003-02-11)
Macroautophagy is an intracellular degradation system for the majority of proteins and some organelles. The molecular mechanism of autophagy has been extensively studied using the yeast, Saccharomyces cerevisiae, during these past 10 years. These studies suggested that the molecular machinery
Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy
Proikas-Cezanne T, et al.
Febs Letters, 581(18), 3396-3404 (2007)
Tassula Proikas-Cezanne et al.
FEBS letters, 581(18), 3396-3404 (2007-07-10)
Autophagy depends on the activity of phosphoinositide-3 kinase class III to generate PI(3)P. We identified the human WIPI protein family of PI(3)P-binding factors and showed that WIPI-1 (Atg18) is linked to autophagy in human cells. Induction of autophagy by rapamycin
WIPI-1alpha(WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy
Proikas-Cezanne T, et al.
Oncogene, 23(58), 9314-9314 (2004)
Tassula Proikas-Cezanne et al.
Oncogene, 23(58), 9314-9325 (2004-12-17)
WD-repeat proteins are regulatory beta-propeller platforms that enable the assembly of multiprotein complexes. Here, we report the functional and bioinformatic analysis of human WD-repeat protein Interacting with PhosphoInosides (WIPI)-1alpha (WIPI49/Atg18), a member of a novel WD-repeat protein family with autophagic
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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