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W4128

Sigma-Aldrich

Williams′ Medium E

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

Williams’ E medium

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Williams′ Medium E, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

L-glutamine: no
phenol red: 0.0107 g/L
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

Williams′ Medium E has been shown to support the growth in long-term culture of adult liver epithelial cells.

Application

Williams′ Medium E has been used to culture primary human hepatocytes and to maintain hepatocellular carcinoma (JHH-6) cell lines.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Florian Douam et al.
PLoS pathogens, 14(3), e1006908-e1006908 (2018-03-06)
Amino-acid coevolution can be referred to mutational compensatory patterns preserving the function of a protein. Viral envelope glycoproteins, which mediate entry of enveloped viruses into their host cells, are shaped by coevolution signals that confer to viruses the plasticity to
Hannah L Paish et al.
Hepatology (Baltimore, Md.), 70(4), 1377-1391 (2019-04-10)
Precision cut liver slices (PCLSs) retain the structure and cellular composition of the native liver and represent an improved system to study liver fibrosis compared to two-dimensional mono- or co-cultures. The aim of this study was to develop a bioreactor
Xiaowen Ma et al.
Liver research, 5(1), 16-20 (2021-08-06)
Mitophagy is a lysosomal degradation pathway that selectively removes damaged, aged and dysfunctional mitochondria. Recent advances in understanding mitophagy highlight its importance in various physiological and pathological conditions including liver diseases. However, reliable quantitative assays to monitor mitophagy in cultured
Wen-Xing Ding et al.
Methods in enzymology, 453, 397-416 (2009-02-17)
Mammalian autophagy has been well characterized in the liver and in hepatocytes. Autophagy plays important roles in the normal physiology of the liver and in the pathogenesis of several liver diseases. This chapter will discuss the commonly used methods for
Dongxin Zhao et al.
PloS one, 4(7), e6468-e6468 (2009-08-04)
The derivation of hepatic progenitor cells from human embryonic stem (hES) cells is of value both in the study of early human liver organogenesis and in the creation of an unlimited source of donor cells for hepatocyte transplantation therapy. Here

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