T7818

Trizma^® Pre-set crystals

BioPerformance Certified, pH 7.5, average M_w 150.6

• Synonyme(s) : Tris crystals
• Code UNSPSC : 12161700
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: BioPerformance Certified
• Forme: crystalline powder
• Poids mol.: average M_w 150.6
• Technique(s): cell culture | mammalian: suitable
• Impuretés: DNase, RNase, Protease, NICKase, none detected; endotoxin, tested; total aerobic microbial count, tested; ≤0.05 A₂₉₀; ≤0.5% water (Karl Fischer); ≤5 ppm Heavy metals (as Pb)
• pH: 7.50 (25 °C, 0.1 M)(lit.); 7.5
• Plage de pH utile: 7.0-9.0
• Solubilité: water: 0.66 g/mL, clear, colorless
• Application(s): diagnostic assay manufacturing

Description

Application

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Autres remarques

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.

Notes préparatoires

Trizma solutions can be autoclaved.

Informations légales

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves