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T5391

Sigma-Aldrich

apo-Transferrine human

γ-irradiated, powder, BioXtra, suitable for cell culture, ≥98%

Synonyme(s) :

Sidérophiline, Transferrine humaine

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About This Item

Numéro CAS:
11096-37-0
Numéro CE :
234-318-8
Numéro MDL:
MFCD00130536
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75

Niveau de qualité

200

Stérilité

γ-irradiated

Gamme de produits

BioXtra

Pureté

≥98%

Forme

powder

Poids mol.

76-81 kDa

Concentration

~25 mM

Technique(s)

cell culture | mammalian: suitable

Impuretés

HIV and HBsAg, source material tested negative
 iron, essentially free

Solubilité

H2O: 50 mg/mL

Numéro d'accès UniProt

P02787

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... TF(7018)

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Description générale

apo-Transferrin (aTF) human can be supplemented with iron or used to bind free iron present in media. It can be used to transport iron into mammalian cells.

Application

apo-Transferrin human has been used as a supplement:
  • in Dulbecco′s modified Eagle medium/HamF12 (DMEM/F-12) to culture human adrenocortical carcinoma cells
  • in DMEM/F-12 to culture neural precursor cells from mice
  • in collecting medium NCSU23 to culture preantral follicles from porcine ovaries

Actions biochimiques/physiologiques

apo-Transferrin (aTF) is an endogenous iron chelator and shows protective effects in the host by scavenging free iron and preventing the formation of toxic reactive oxygen species (ROS). It shows a therapeutic effect against thalassemia. After the release of iron, receptor-bound apo-transferrin reaches the cell surface instead of being transported to lysosomes for degradation. The cell surface has a neutral pH that promotes the release of apo-transferrin from its receptor, where it can again circulate and bind additional iron to undergo further rounds of iron delivery to cells.

Autres remarques

Protéine de transport du fer du type non-hème.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

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Miwako Yamamiya et al.
Biochemical and biophysical research communications, 513(4), 841-845 (2019-04-21)
Microglia are central nervous system-resident immune cells that play a crucial role in brain development by interacting with neural precursor cells (NPCs). It has been reported that microglia regulate the number of NPC by phagocytosis, inducing apoptosis, and promoting proliferation.
Hiroshi Kawabata
Free radical biology & medicine, 133, 46-54 (2018-07-04)
In vertebrates, transferrin (Tf) safely delivers iron through circulation to cells. Tf-bound iron is incorporated through Tf receptor (TfR) 1-mediated endocytosis. TfR1 can mediate cellular uptake of both Tf and H-ferritin, an iron storage protein. New World arenaviruses, which cause
Miwako Yamamiya et al.
Biochemical and biophysical research communications, 513(4), 841-845 (2019-04-21)
Microglia are central nervous system-resident immune cells that play a crucial role in brain development by interacting with neural precursor cells (NPCs). It has been reported that microglia regulate the number of NPC by phagocytosis, inducing apoptosis, and promoting proliferation.
Arunima Purkayastha et al.
Cell stem cell, 27(6), 869-875 (2020-12-02)
Current smoking is associated with increased risk of severe COVID-19, but it is not clear how cigarette smoke (CS) exposure affects SARS-CoV-2 airway cell infection. We directly exposed air-liquid interface (ALI) cultures derived from primary human nonsmoker airway basal stem
Heather Etchevers
Nature protocols, 6(10), 1568-1577 (2011-10-01)
A highly enriched population of neural crest cells (NCCs) from amniote embryos, such as from chicks, mice and humans, is desirable for experiments in fate determination. NCCs are also useful for testing the functional effects of molecular changes underlying numerous
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Articles

Transferrin in Cell Culture

How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.

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