SRP5127

RhoA, His tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonyme(s) : ARH12, ARHA, RHO12, RHOH12
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥70% (SDS-PAGE)
• Forme: buffered aqueous glycerol solution
• Poids mol.: ~23 kDa
• Numéro d'accès NCBI: NM_001664
• Application(s): cell analysis
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... RHOA(387)

Description

Description générale

Ras homolog family member A (RhoA) is a small GTPase which is part of the Rho gene family. The gene encoding it is localized on human chromosome 3p21.31.

Application

RhoA, His tagged human has been used for in vitro kinase assay.

Actions biochimiques/physiologiques

Ras homolog family member A (RhoA) interacts with protein kinases and serves as a target of activated GTPase. RhoA plays an important role in the initial events of cell protrusion. Rho mediates corneal epithelial migration in response to external stimuli by regulating the organization of the actin cytoskeleton. The inhibition of RhoA signaling is required for both vaccinia morphogenesis and virus-induced cell motility. Vaccinia inhibits RhoA signaling using the viral protein F11 and this can enhance the spread of infection. The protein has a role in basal body docking and planar polarization.

Forme physique

Supplied in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Notes préparatoires

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H360D
• Conseils de prudence: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable