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SRP5009

Sigma-Aldrich

CDK1/CyclinB1, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonyme(s) :

CCNB, CCNB1, CDC2

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Produit recombinant

expressed in baculovirus infected Sf9 cells

Gamme de produits

PRECISIO® Kinase

Pureté

≥70% (SDS-PAGE)

Forme

buffered aqueous glycerol solution

Activité spécifique

12-16 nmol/min·mg

Poids mol.

~58 kDa (CDK1)
 ~75 kDa (CyclinB1)

Numéro d'accès NCBI

NM_001786
NM_031966

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... CCNB1(891) , CDK1(983)

Description générale

The cyclin B1 (CCNB1) gene is mapped to human chromosome 5q13.2. The gene codes for a 62kDa protein containing a classical cyclin box domain. CCNB1 shuttles between the nucleus and cytoplasm until prophase begins. It is a member of the cyclin family and is expressed at low levels in normal cells. CDK1 (cyclin-dependent kinase 1) belongs to the Ser/Thr protein kinase family. The gene encoding it is localized on human chromosome 10q21.

Actions biochimiques/physiologiques

The cyclin B1 (CCNB1) and cyclin-dependent kinase 1 (CDK1) complex plays a crucial role in the regulation of mitosis. Elevated expression of CCNB1 has been observed in various types of human cancers including breast, lung, colon, prostate and head and neck cancers. Thus, this protein can be considered as a potent target for cancer vaccines.In addition, CCNB1 also serves as a prognostic biomarker for estrogen-receptor-positive (ER+) breast cancer. CDK1 (cyclin-dependent kinase 1) controls various mitotic processes like reorganization of the cytoskeleton and segregation of chromosomes. It is essential for the multiplication of mammalian cells.

Forme physique

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Notes préparatoires

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Informations légales

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Chk1-induced CCNB1 overexpression promotes cell proliferation and tumor growth in human colorectal cancer
Yifeng Yang
Cancer Biology & Therapy (2014)
The Tumor Antigen Cyclin B1 Hosts Multiple CD4 T Cell Epitopes Differently Recognized by Pre-Existing Naive and Memory Cells in Both Healthy and Cancer Donors
Claire Chevaleyre
Journal of Immunology, 195(4) (2015)
CCNB1 is a prognostic biomarker for ER+ breast cancer
Kun Ding
Medical Hypotheses, 83(3) (2014)
Expression analysis of mitotic spindle checkpoint genes in breast carcinoma: role of NDC80/HEC1 in early breast tumorigenicity, and a two-gene signature for aneuploidy
Ivan B
Molecular Cancer (2011)
Soraya Játiva et al.
Cellular and molecular life sciences : CMLS, 76(18), 3601-3620 (2019-03-31)
Exit from mitosis and completion of cytokinesis require the inactivation of mitotic cyclin-dependent kinase (Cdk) activity. In budding yeast, Cdc14 phosphatase is a key mitotic regulator that is activated in anaphase to counteract Cdk activity. In metaphase, Cdc14 is kept
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