SRP0127

DNMT2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Synonyme(s) : DNA (cytosine-5-)-methyltransferase 2, PUMET, RNMT1, tRNA (cytosine-5-)-methyltransferas, tRNA aspartic acid methyltransferase 1
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Essai: ≥80% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 71 kDa
• Conditionnement: pkg of 10 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_004412
• Numéro d'accès UniProt: Q6ICS7
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... TRDMT1(1787)

Description

Description générale

DNA (cytosine-5-)-methyltransferase 2 (DNMT2) is a highly conserved protein which is part of the DNA methyltransferase family. It possesses a conserved cysteine residue in its catalytic pocket. The gene encoding it is localized on human chromosome 10p14->p12.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

DNA (cytosine-5-)-methyltransferase 2 (DNMT2) methylates the cytosine-38 residue of the aspartic acid transfer RNA (tRNA-Asp). It has been shown to interact with the anticodon stem and loop. The protein also functions in cellular physiology and stress response. It is significantly expressed in cancers.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.