SRP0120

Sirtuin 6 human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonyme(s) : NAD-dependent ADP-ribosyltransferase sirtuin-6, SIR2L6, SIRT6, sir2-like 6
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥70% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 65 kDa
• Conditionnement: pkg of 100 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_016539
• Numéro d'accès UniProt: Q8N6T7
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... SIRT6(51548)

Description

Description générale

Sirtuin 6 (Sir6) is encoded by the gene mapped to human chromosome 19p13.3, a region linked with development of acute leukemia. Sir 6 is mainly expressed in bone cells and in the ovaries, but it is absent in the bone marrow. Sirt6 is a chromatin-associated protein localized specifically in nucleus.
Human Sirtuin 6, GenBank Accession No. NM_016539), full length with N-terminal ST tag, MW = 65kDa, expressed in Escherichia coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

Sirtuin 6 (Sir6) is a mono-ADP-ribosyltransferase, which catalyzes the transfer of ADP-ribose moiety from NAD to itself and histones. It has been implicated in the regulation of DNA repair, genomic stability and telomere integrity, gene expression, and metabolism. In addition, it also prevents age-related disorders and premature aging. Sir6 plays a vital role in the inhibition of nuclear factor-κB (NF-κB)–mediated inflammatory responses in rheumatoid joints. Hence, Sir6 is considered to be a potent target for treatment of rheumatoid arthritis (RA). Sir6 is also majorly implicated in the regulation of glucose and fat metabolism. Sir6 prevent cardiac hypertrophy in vitro via inhibiting NF-κB-dependent transcriptional activity and this effect is relied on its deacetylase activity.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.