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Key Documents

SRP0109

Sigma-Aldrich

HDAC-7 human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonyme(s) :

HD7, HD7A, HDAC7A, Histone deacetylase 7

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in baculovirus infected insect cells

Pureté

≥70% (SDS-PAGE)

Forme

aqueous solution

Puissance

≥10 units/mg

Poids mol.

78 kDa

Conditionnement

pkg of 2 and 10 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Concentration

>0.02 mg/mL

Numéro d'accès NCBI

AY302468

Numéro d'accès UniProt

Q8WUI4

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... HDAC7(51564)

Description générale

HDAC7 (histone deacetylase 7) is located on human chromosome 12q13. It is expressed in fetal and adult pre-B cells.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

Histone deacetylases (HDACs) play crucial roles in the epigenetic modulation of gene expression and helps in the growth, differentiation, and apoptosis of cancer cells. Knockdown of HDAC7 stimulates apoptosis and autophagy in salivary mucoepidermoid carcinoma cells. It may play a vital role in both types of B lymphopoiesis. HDAC7 serves as a repressor of myeloid genes.

Définition de l'unité

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Histone deacetylase 7 silencing induces apoptosis and autophagy in salivary mucoepidermoid carcinoma cells.
Ahn MY & Yoon JH
Journal of Oral Pathology & Medicine, 46(4), 276-283 (2017)
Overview of the classical histone deacetylase enzymes and histone deacetylase inhibitors.
Ververis K & Karagiannis TC
ISRN Cell Biology , 2012 (2012)
Bruna Barneda-Zahonero et al.
PLoS genetics, 9(5), e1003503-e1003503 (2013-05-23)
B lymphopoiesis is the result of several cell-commitment, lineage-choice, and differentiation processes. Every differentiation step is characterized by the activation of a new, lineage-specific, genetic program and the extinction of the previous one. To date, the central role of specific
Caihua Zhu et al.
Journal of molecular medicine (Berlin, Germany), 89(3), 279-289 (2010-12-02)
Histone deacetylases (HDACs) play fundamental roles in the epigenetic regulation of gene expression and contribute to the growth, differentiation, and apoptosis of cancer cells. Although HDACs are recognized to be closely related to cancer development and altered expression of certain

Articles

Histone Modification and Chromatin Remodeling | Epigenetics

Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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