SAE0023

Alanine Racemase from Bacillus stearothermophilus

buffered aqueous solution, ≥10 IU/mg solid

• Synonyme(s) : Alanine Racemase from Bacillus stearothermophilus
• Numéro CAS: 9024-06-0
• Numéro de classification (Commission des enzymes): 5.1.1.1 (BRENDA, IUBMB)
• Numéro MDL: MFCD03454300
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Bacillus sp. (B. stearothermophilus)
• Niveau de qualité: 200
• Forme: buffered aqueous solution
• Activité spécifique: ≥10 IU/mg solid
• Poids mol.: M_r 78 kDa (2 subunits 39 kDa each)
• Température de stockage: −20°C

Description

Application

Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-¹⁴C]alanine to a racemic mixture of L/D- [¹⁴C]alanine †.

Actions biochimiques/physiologiques

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†

Définition de l'unité

One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.

Forme physique

Aqueous solution containing 10 mM potassium phosphate

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable