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Key Documents

SAB4700630

Sigma-Aldrich

Monoclonal Anti-5-bromodeoxyuridine antibody produced in mouse

clone MoBu-1, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

Anti-BrdU

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

MoBu-1, monoclonal

Forme

buffered aqueous solution

Concentration

1 mg/mL

Technique(s)

flow cytometry: suitable

Isotype

IgG1

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Description générale

The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU).

Immunogène

5-bromodeoxyuridine conjugated with hemocyanine

Application

Monoclonal Anti-5-bromodeoxyuridine antibody produced in mouse has been used in Immunocytochemistry. The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 1-2 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Actions biochimiques/physiologiques

5-Bromodeoxyuridine (BrdU) is an analogue of thymidine, used to measure DNA synthesis immunochemically in living cells. BrdU along with cAMP and butyrate plays a vital role in the cellular differentiation in several mammalian cell types. It also inhibits cell growth in mammalian and yeast cells by modulating the expression of particular genes involved in cell division. In the absence of DNA synthesis, brdu promotes mouse neuroblastoma C1300 cell differentiation into cells that morphologically are similar to mature neurons. BrdU activates cell senescence-like phenomenon in mammalian cells.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

D Stanek et al.
European journal of cell biology, 79(3), 202-207 (2000-04-25)
The available data concerning the subnucleolar localisation of the individual steps of precursor-ribosomal RNA (pre-rRNA) processing are ambiguous. According to in situ hybridisation studies, the late steps of pre-rRNA processing have been located into the granular component of the nucleolus
Alessio Ligabue et al.
PloS one, 7(10), e47318-e47318 (2012-10-12)
5-fluorouracil, a commonly used chemotherapeutic agent, up-regulates expression of human thymidylate synthase (hTS). Several different regulatory mechanisms have been proposed to mediate this up-regulation in distinct cell lines, but their specific contributions in a single cell line have not been
Overexpression of HAM1 gene detoxifies 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae.
Takayama S, et al.
Current Genetics, 52(5-6), 203-211 (2007)
AT-hook proteins stimulate induction of senescence markers triggered by 5-bromodeoxyuridine in mammalian cells.
Satou W, et al.
Experimental Gerontology, 39(2), 173-179 (2004)
5-bromodeoxyuridine-induced differentiation of a neuroblastoma.
Schubert D and Jacob F.
Proceedings of the National Academy of Sciences of the USA, 67(1), 247-254 (1970)

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