SAB4200841

Anti-Syntaxin antibody, Mouse monoclonal

clone HPC-1, purified from hybridoma cell culture

• Synonyme(s) : Anti-STX1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Forme d'anticorps: purified from hybridoma cell culture
• Niveau de qualité: 200
• Clone: HPC-1, monoclonal
• Forme: liquid
• Espèces réactives: monkey, rat, human, dog, mouse, bovine
• Concentration: ~1 mg/mL
• Technique(s): immunoblotting: 0.25-0.5 μg/mL using human neuroblastoma SHSY5Y cell lysate.
• Isotype: IgG1
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Syntaxins are a family of membrane-integrated proteins that are instrumental in exocytosis of vesicles.6 The syntaxin family consists of 15 genes in mammals and 7 in yeast, and there are syntaxin-like sequences in all eukaryotes examined.7 All mammalian syntaxins, with the exception of syntaxin 11, are transmembrane proteins anchored by their C-terminal tails.7
Syntaxin-1(also known as HPC-1 antigen or Stx1),8-9 a 35 kDa molecule with carboxyl-terminal membrane anchor, is a synaptic protein identified by its ability to interact with the synaptic vesicle protein synaptotagmin

Spécificité

Monoclonal Anti-Syntaxin antibody specifically recognizes membrane protein syntaxin, expressed predominantly in neuronal tissues.2,3 The antibody reacts with syntaxin from human, mouse and rat origin. The antibody has also been used to isolate syntaxin cDNA clones by screening a λgt 11 library from rat hippocampus.3

Application

The antibody may be used in various immunochemical techniques including Immunoblotting (~35 kDa), Immunohistochemistry4 and Immunofluorescence5.

Actions biochimiques/physiologiques

Syntaxin-1 protein is an essential component of the synaptic vesicle soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex and is essential for neurotransmission.10 It forms a four-helical bundle complex with proteins SNAP-25 and VAMP2 that drives fusion of vesicular and plasma membranes.6,11

Forme physique

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Clause de non-responsabilité

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg