SAB4200796
Anti- Bovine IgG-Peroxidase antibody, Mouse monoclonal
clone BG-18, purified from hybridoma cell culture
Synonyme(s) :
Bovine immunoglobulin G
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
peroxidase conjugate
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
BG-18, monoclonal
Forme
lyophilized powder
Espèces réactives
bovine
Concentration
~2 mg/mL
Technique(s)
direct ELISA: 1:20,000-1:40,000 using 1 μg/ml bovine IgG for coating
Isotype
IgG1
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Description générale
Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the BG-18 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with the purified bovine IgG.
Immunoglobulin G (IgG) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.
Immunoglobulin G (IgG) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.
The bovine immunoglobulin system closely resembles that of other mammalian species with respect to the physiochemical properties and nomenclature. The main bovine IgG class antibodies are IgG1 and IgG2, which have antigenic differences in the Fc region of the heavy chains.4
Spécificité
Monoclonal Anti-Bovine IgG specifically recognizes an epitope on the heavy chain of both bovine IgG1 and IgG2. The antibody shows no cross-reactivity with bovine IgM or IgG from the following species: human, goat, sheep, rabbit, pig, dog, chicken, cat, guinea pig or horse.
Immunogène
Purified bovine IgG
Application
The antibody is recommended to use in various immunological techniques, including ELISA1, Immunohistochemistry2 and Immunobloting3
Actions biochimiques/physiologiques
Different immunoglobulin classes and subclasses (isotypes) perform distinctive effector functions. Therefore, the ability to characterize antibody isotype is fundamental to the analysis of the immune response. The diversity of immunoglobulin isotypes associated with the immunity pattern serves as an indicator indistinguishing the phase of infection with various agents.
Forme physique
Supplied as a lyophilized powder.
Stockage et stabilité
Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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S S Saini et al.
Scandinavian journal of immunology, 65(1), 32-38 (2007-01-11)
Analysis of the heavy-chain gene (pTGHC9907) encoding a bovine IgG1 antibody against bovine herpes virus type 1 (BHV-1) isolated from a Holstein cow has led to the identification of a new IgG1 sequence allele. A comparison of nucleotide sequence of
Use of sandwich IgG ELISA for the detection and quantification of adulteration of milk and soft cheese
Ian P.Hurley
International dairy journal, 16, 805-812 (2006)
Histological and Immunohistochemical Studies on the Furstenberg's Rosette in Cows.
Asti RN
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 17, 223-228 (2011)
A C Rodrigues da Silva et al.
Revista do Instituto de Medicina Tropical de Sao Paulo, 42(2), 95-98 (2000-05-16)
Despite the absence of current official reports showing the number of cattle infected by rabies, it is estimated that nearly 30,000 bovines are lost each year in Brazil. In order to minimize the important economic losses, control of the disease
Cheryl Jenkins et al.
Parasites & vectors, 9, 106-106 (2016-02-26)
Bovine theileriosis caused by Theileria orientalis is an emerging disease of cattle in the Asia-Pacific region where it causes a significant economic burden to meat and milk production. While host immunological responses to the lymphocyte-transforming species of Theileria, T. parva
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