Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

SAB4200755

Sigma-Aldrich

Anti-Human IgE-Peroxidase antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

Synonyme(s) :

Anti-Immunoglubulin E

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Niveau de qualité

200

Conjugué

peroxidase conjugate

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

GE-1, monoclonal

Forme

lyophilized powder

Espèces réactives

human

Technique(s)

ELISA: 1:20,000-1:30,000 using 5 μg/mL Human IgE myeloma for coating

Isotype

IgG2b

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgE myeloma protein. IgE (Immunoglobulin E) is the least abundant immunoglobulin isotype. IgE is produced by plasma cells and is highly functional in the lung, skin, and mucous membranes. Normal levels of IgE in serum are less than 1 μmL.

Spécificité

Monoclonal Anti-Human IgE specifically recognizes Human IgE. The antibody shows no cross-reactivity with human IgG1, IgG2, IgG3, IgG4, IgA, and IgM.

Immunogène

Purified human IgE

Application

Anti-Human IgE-Peroxidase antibody has been used in enzyme linked immunosorbent assay (ELISA).

Actions biochimiques/physiologiques

IgE plays a critical role in allergic reactions and host protection from parasites. During an allergic reaction such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease, or primary immunodeficiency the serum IgE levels can be induced up to 5-fold. IgE specifically and reversibly binds to the membrane receptor Fc-epsilon receptors (FcεR) type I on the surface of basophils, eosinophils, and mast cells. This binding activates the release of histamine, leukotrienes, and other mediators responsible for hypersensitivity. The recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.

Forme physique

Supplied as a lyophilized powder.

Autres remarques

This product is for R&D use only, not for drug, household, or other uses.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
047M4811V
51170404

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

IgE and mast cells in allergic disease
Galli S J and Tsai M
Nature Medicine, 18(5), 693-693 (2012)
IgE and mast cells in allergic disease
Wachholz P A, et al.
Journal of Immunotoxicology, 1(3-4), 189-199 (2005)
R H Buckley et al.
The Journal of clinical investigation, 55(1), 157-165 (1975-01-01)
Concentrations of IgD and IgE were measured in sera from 165 patients with well-defined immunodeficiency in an effort to find information possibly relevant to the roles of antibodies of these classes in host defense. Values for both immunoglobulins were generally
F Zellweger et al.
Allergy, 71(12), 1652-1661 (2016-10-07)
IgE-associated allergic diseases belong to the most common inflammatory conditions. Their clinical manifestation ranges from mild symptoms to life-threatening episodes. Often patients experience a reduction in physical and psychologic well-being and suffer from a decreased quality of life due to
T J Suutari et al.
Journal of investigational allergology & clinical immunology, 16(5), 296-302 (2006-10-17)
Allergic reactions to cow's milk are common in small children. One of the main protein allergens found in cow's milk is beta-lactoglobulin (beta-Lg). Reindeer and bovine milk both contain related beta-Lg proteins, but the allergenicity of reindeer beta-Lg has not
Afficher tous les articles scientifiques apparentés

Articles

Anti-Human Antibodies for COVID-19 (SARS-CoV-2) Immunoglobulin Detection Research

Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique