SAB4200739
Anti-Protein kinase C antibody, Mouse monoclonal
clone MC5, purified from hybridoma cell culture
Synonyme(s) :
Anti-PKC
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.44
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
MC5, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~80 kDa
Espèces réactives
chicken, human, fish, mouse, bovine, rabbit, rat
Concentration
~1.0 mg/mL
Technique(s)
immunoblotting: 1.25-2.5 μg/mL using human bone marrow neuroblast SH-SY5Y cell extract
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
Isotype
IgG2a
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PRKCA(5578)
Description générale
Anti-Protein kinase C antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the MC5 hybridoma produced by the fusion of X-63 myeloma cells and splenocytes from a BALB/c mouse immunized with purified bovine brain protein kinase C1. Protein kinase C (PKC), is a family of phospholipid- and calcium-activated, diacylglycerol (DAG)-dependent serine/threonine kinases.
Spécificité
Monoclonal Anti-Protein kinase C antibody recognizes an epitope located within the amino acid residues 296- 317, at the hinge region, close to or at the trypsin cleavage site of Protein kinase C molecule . The antibody specifically recognizes Protein kinase C from human, rat, bovine, mouse, chicken, rabbit and fish origin.
Application
Anti-Protein kinase C antibody has been used in:
- immunoblotting
- immunohistochemistry
- immunofluorescence
- immunoprecipitation
Actions biochimiques/physiologiques
Protein kinase C (PKC) play a central role in a variety of cell processes, including: intracellular signal transduction, cell proliferation, apoptosis, differentiation, migration and adhesion, tumor promotion, cardiac hypertrophy, angiogenesis, platelet function, inflammation and oncogenesis. Neuronal PKC modulates several functions such as nerve cell excitability, neuronal plasticity and release of neurotransmitters. PKC activity is regulated by two mechanisms- firstly by phosphorylation which regulates the active site and subcellular localization of the enzyme, secondly by secondary messengers which facilitate association of PKC on membrane. The PKC activity is strongly associated with tumor initiation and progression and have been implemented as a target for treatment of cancer progression and diabetic retinopathy.
Forme physique
Purified bovine brain protein kinase C
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
S C Kiley et al.
The Journal of biological chemistry, 266(35), 23761-23768 (1991-12-15)
GH4C1 cells, which express Ca(2+)-dependent alpha- and beta- as well as Ca(2+)-independent gamma-, epsilon- and zeta-protein kinase C (PKC) isozymes, provide a cell culture model for studying isozyme-specific properties and functions. Hormonal activation of PKCs regulates the differentiated functions of
R Garg et al.
Oncogene, 33(45), 5225-5237 (2013-12-18)
Since their discovery in the late 1970s, protein kinase C (PKC) isozymes represent one of the most extensively studied signaling kinases. PKCs signal through multiple pathways and control the expression of genes relevant for cell cycle progression, tumorigenesis and metastatic
Protein kinase C (PKC) isozymes and cancer
Kang J H
New Journal of Science, 2014 (2014)
T Ng et al.
Science (New York, N.Y.), 283(5410), 2085-2089 (1999-03-26)
Spatially resolved fluorescence resonance energy transfer (FRET) measured by fluorescence lifetime imaging microscopy (FLIM), provides a method for tracing the catalytic activity of fluorescently tagged proteins inside live cell cultures and enables determination of the functional state of proteins in
Inhibition of protein kinase C enhances angiogenesis induced by platelet-derived growth factor C in hyperglycemic endothelial cells
Moriya J and Ferrara N
Cardiovascular Diabetology, 14(1), 19-19 (2015)
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