SAB4200715

Anti-β-tubulin antibody, Mouse monoclonal

clone 2-28-33, purified from hybridoma cell culture

• Synonyme(s) : Anti-TUBB1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: 2-28-33, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: ~50 kDa
• Espèces réactives: dog, chicken, rat, bovine, monkey, human, mouse
• Concentration: ~1.0 mg/mL
• Technique(s): immunoblotting: 0.5 μg/mL using mouse embryo fibroblast NIH-3T3 cells extract; immunofluorescence: 1-2 μg/mL using human foreskin fibroblast HS68 cells; immunohistochemistry: suitable
• Isotype: IgG1
• Numéro d'accès UniProt: P07437
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TUBB(203068) ; mouse ... Tubb3(22152)

Description

Description générale

Anti-β-tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 2-28-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin also known as TUBB is the major building block of microtubules. Microtubules assemble from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of ~ 50 kDa and share considerable homology. For β-tubulin, five evolutionarily conserved isotypes have been identified, which are regularly utilized in the same cell types of different species.

Immunogène

Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Application

Monoclonal Anti-β-tubulin has been used:

• in immunoblotting
• in immunofluorescence
• in immunohistochemistry

Actions biochimiques/physiologiques

Monoclonal Anti-β-tubulin specifically recognizes the two major β-tubulin isotypes and one of the minor β-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans. It also binds to Sarkosyl resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, neuronal axons of the larva of Ciona intestinalis (ascidian), microtubules in root tip cells in Arabidopsis thaliana and to human, mouse, rat, monkey, bovine, dog and chicken β-tubulin. The different β-tubulin isotypes are suggested to differ from each other in their ability to polymerize into microtubules.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable