SAB4200273

Anti-Orai1 antibody, Mouse monoclonal

clone ORAI1-89, purified from hybridoma cell culture

• Synonyme(s) : Monoclonal Anti-CRACM1, Monoclonal Anti-ORAI calcium release-activated calcium modulator 1, Monoclonal Anti-ORAT1, Monoclonal Anti-TMEM142A, Monoclonal Anti-calcium release-activated calcium channel protein 1, Monoclonal Anti-transmembrane protein 142A
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: ORAI1-89, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~30 kDa (non-glycosylated Orai1); antigen ~50 kDa (glycosylated Orai1)
• Espèces réactives: human
• Concentration: ~1.0 mg/mL
• Technique(s): immunoprecipitation (IP): suitable; indirect immunofluorescence: suitable; western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human Orai1.
• Isotype: IgG1
• Numéro d'accès UniProt: Q96D31
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ORAI1(84876)

Description

Description générale

Monoclonal Anti-Orai1 (mouse IgG1 isotype) is derived from the hybridoma ORAI1-89 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide located near the C-terminus of human Orai1, conjugated to KLH. Orai1, also called Calcium release-activated calcium channel protein 1 (CRACM1), is an evolutionary conserved plasma membrane protein. Orai1 is predicted to contain four transmembrane domains with its N- and C-termini in the cell cytoplasm.

ORAI calcium release-activated calcium modulator 1 (Orai1), also known as transmembrane protein 142A (TMEM142A), is encoded by the gene mapped to human chromosome 12q24.31.{49}. The encoded protein contains four transmembrane helices with highly conserved glutamate residues in transmembrane helices 1 and 3.

Immunogène

synthetic peptide located near the C-terminus of human Orai1, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse.

Application

Monoclonal Anti-Orai1 antibody has been used in

• immunoblotting,
• immunofluorescence
• immunoprecipitation
• proximity ligation assay.

Monoclonal Anti-Orai1 antibody produced in mouse has been used in proximity ligation assay.

Actions biochimiques/physiologiques

Orai1 essential for store-operated calcium entry in T cells and fibroblasts. Store-operated calcium entry is mediated by Ca²⁺ release-activated Ca²⁺ (CRAC) channels, following Ca²⁺ depletion from the endoplasmic reticulum (ER) stores, caused by stimulation of immune cells. This process is crucial for gene transcription, proliferation and cytokine release. Mutations in two highly conserved glutamate residues, E¹⁰⁶ and E¹⁹⁰, diminish Ca²⁺ influx and promote changes in ion selectivity, providing strong evidence that Orai is a pore subunit of the CRAC channel. Orai1 co-localizes with STIM1 (stromal interaction molecule 1) near the plasma membrane after store depletion. A point mutation in Orai1 is responsible for the genetic defect in store-operated Ca²⁺ entry and I-CRAC in cells of the hereditary severe combined immune deficiency (SCID) syndrome patients.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable