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Key Documents

SAB2700181

Sigma-Aldrich

Anti-NS3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Dengue virus 2 Nonstructural protein NS3, Non-structure protein 3

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

Dengue virus

Technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: 500-3000

Numéro d'accès NCBI

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Non-structure protein 3 (NS3), also known as dengue virus type 2 (DEN2), belongs to the Flaviviridae family. NS3 is a multifunctional protein with a molecular mass of about 69 kDa. It contains serine protease domain (NS3-pro) and it needs the hydrophilic domain of NS2B (NS2BH) for activation.

Immunogène

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 354 and 568 of NS3 (Dengue virus 2) according to NP_739587

Application

Suggested starting dilutions are as follows: FACS: Assay-dependent dilution, ICC/IF: 1:100-1:2000, IHC: Assay-dependent dilution, IHC-P: Assay-dependent dilution, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Anti-NS3 antibody produced in rabbit has been used in flow cytometry.

Actions biochimiques/physiologiques

Non-structure protein 3 (NS3) along with NS2B play a vital role in proteolytic cleavage of dengue virus (DEN) non-structural proteins. NS3 also acts as a RNA-stimulated nucleoside-triphosphatase (NTPase)/RNA helicase and a 5′-RNA triphosphatase (RTPase).

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Aquatic Chronic 3 - Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins.
Falgout B, et al.
Journal of Virology, 65(5), 2467-2475 (1991)
RIG-I?like Receptor Triggering by Dengue Virus Drives Dendritic Cell Immune Activation and TH1 Differentiation.
Sprokholt JK, et al.
Journal of Immunology, 1602121-1602121 (2017)
RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody production.
Sprokholt JK, et al.
PLoS Pathogens, 13(11), e1006738-e1006738 (2017)
Modulation of the nucleoside triphosphatase/RNA helicase and 5′-RNA triphosphatase activities of Dengue virus type 2 nonstructural protein 3 (NS3) by interaction with NS5, the RNA-dependent RNA polymerase.
Yon C, et al.
The Journal of Biological Chemistry (2005)
Joris K Sprokholt et al.
PLoS pathogens, 13(11), e1006738-e1006738 (2017-12-01)
Follicular T helper cells (TFH) are fundamental in orchestrating effective antibody-mediated responses critical for immunity against viral infections and effective vaccines. However, it is unclear how virus infection leads to TFH induction. We here show that dengue virus (DENV) infection

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