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Key Documents

SAB1406364

Sigma-Aldrich

Anti-RAD51 antibody produced in mouse

purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

BRCC5, HRAD51, HsRad51, HsT16930, RAD51A, RECA

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~37 kDa

Espèces réactives

rat, mouse, human

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: 1 μg/mL

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... RAD51(5888)

Description générale

The protein encoded by this gene is a member of the RAD51 protein family. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) The RAD51 gene is located on human chromosome 15q15.1.

Immunogène

RAD51 (NP_002866.2, 1 a.a. ~ 339 a.a) full-length human protein.

Sequence
MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Anti-RAD51 antibody produced in mouse has been used in Western blot analysis and Immunohistochemistry.

Actions biochimiques/physiologiques

RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein replication protein A (RPA) and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage.
RAD51 specifically repairs double stranded breaks in the DNA. Upregulation of RAD51 is observed in a number of tumors and indicates poor prognosis. Rad51 induces G0/G1 to S phase transition in cell cycle. Therefore, inhibition of RAD51 reduces the progression of cancer in cases such as cervical cancer.

Forme physique

Solution in phosphate buffered saline, pH 7.4

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA.
Golub E I, et al.
Nucleic Acids Research, 26(23), 5388-5393 (1998)
The protein PprI provides protection against radiation injury in human and mouse cells.
Yi S, et al.
Scientific Reports, 6, 26664-26664 (2016)
Role of BRCA1 and BRCA2 as regulators of DNA repair, transcription, and cell cycle in response to DNA damageRole of BRCA1 and BRCA2 as regulators of DNA repair, transcription, and cell cycle in response to DNA damage.
Kiyotsugu Y and Yoshio M
Cancer Science, 95(11), 866-871 (2004)
The homologous recombination protein RAD51 is a promising therapeutic target for cervical carcinoma.
Qian C, et al.
Oncology Reports, 38(2), 767-774 (2017)
Jia Hu et al.
PloS one, 8(8), e72104-e72104 (2013-08-27)
XRCC3 and RAD51 are two important members in homologous recombination repair pathway. This study was performed to detect the expressions of these two molecules in breast cancer and explore their correlations with clinicopathological factors. Immunohistochemistry was used to detect protein

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