S6072

Anti-STIM1 (N-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-GOK, Anti-Stromal interaction molecule I
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 90 kDa
• Espèces réactives: rat, mouse, human
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Concentration: ~1 mg/mL
• Technique(s): immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells.; indirect immunofluorescence: 5-10 μg/mL using human HeLa cells.; western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells.
• Numéro d'accès UniProt: Q13586
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... STIM1(6786) ; mouse ... Stim1(20866) ; rat ... Stim1(117086)

Description

Description générale

STIM1 (stromal interaction molecule 1) is a conserved single-pass transmembrane protein, which is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 is modified by phosphorylation and N-linked glycosylation. STIM1 localized predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca²⁺ levels.

Immunogène

synthetic peptide corresponding to amino acids 61-74 of human STIM1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Application

Anti-STIM1 (N-terminal) antibody produced in rabbit has been used in:

• western blotting
• immunoprecipitation
• immunofluorescence

Actions biochimiques/physiologiques

STIM1 (stromal interaction molecule 1) is required for the activation of store-operated Ca²⁺ influx. Overexpression of STIM1 and Orai1 markedly increases the calcium release-activated calcium (CRAC) current (I-CRAC).

Forme physique

Solution in 0.01 M phosphate buffered saline, 7.4 pH, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)