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Key Documents

S500HR

Sigma-Aldrich

Sephacryl® Size Exclusion Resin

500-HR, MW range 40-20,000 kDa (dextrans)

Synonyme(s) :

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

Numéro CAS:
65546-95-4
Numéro MDL:
MFCD00165824
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

product name

Sephacryl®, 500-HR, MW range 40-20,000 kDa (dextrans)

Description

stability: stable to 0.2M NaOH

Forme

suspension (20% aqueous ethanol)

Caractéristiques

autoclavable

Matrice

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

Taille des particules

25-75 μm (wet)

Dimension de pores

40-20,000 kDa MW range (dextrans)

Compatibilité

mode of use size exclusion chromatography

Température de stockage

2-8°C

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Description générale

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S500HR-750ML′s updated product number is GE17-0613-01

Application

Sephacryl® has been used in size exclusion chromatography purification of the Acacia tortuosa gum, dextran-RBA12 from Weissella cibaria and dextran from Weissella cibaria JAG8.
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Informations légales

Sephacryl is a registered trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

100.4 - 109.4 °F - closed cup

Point d'éclair (°C)

38 - 43 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

M A Karymov et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(14), 2631-2641 (2001-12-01)
Dynamic alterations in chromatin structure mediated by postsynthetic histone modifications and DNA methylation constitute a major regulatory mechanism in DNA functioning. DNA methylation has been implicated in transcriptional silencing, in part by inducing chromatin condensation. To understand the methylation-dependent chromatin
Theresa L Gioannini et al.
The Journal of biological chemistry, 277(49), 47818-47825 (2002-10-10)
Experiments utilizing endotoxin aggregates, lipooligosaccharides (LOS) isolated from metabolically labeled Neisseria meningitidis serotype group B, demonstrate that albumin is an essential component of lipopolysaccharide binding protein- (LBP) and sCD14-dependent 1) disaggregation of LOS and 2) LOS activation of human umbilical
Structural and biocompatibility properties of dextran from Weissella cibaria JAG8 as food additive
Tingirikari JM, et al.
International Journal of Food Sciences and Nutrition, 65(6), 686-691 (2014)
Xiaolei Wang et al.
Molecular microbiology, 83(1), 195-207 (2011-11-25)
Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set
Takashi Ideue et al.
Genes & development, 21(16), 1993-1998 (2007-08-07)
Pre-mRNA splicing specifically deposits the exon junction complex (EJC) onto spliced mRNA, which is important for downstream events. Here, we show that EJC components are primarily recruited to the spliceosome by association with the intron via the intron-binding protein, IBP160.
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