RAB0105

Human Cystatin C ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonyme(s) : Cystatin-3, Cystatin-C
• Code UNSPSC : 41116158
• Nomenclature NACRES : NA.32

Propriétés

• Espèces réactives: human
• Conditionnement: kit of 96 wells (12 strips x 8 wells)
• Technique(s): ELISA: suitable; capture ELISA: suitable
• Entrée: sample type plasma; sample type urine; sample type serum; sample type cell culture supernatant(s)
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 300 pg/mL; standard curve range: 0.313-20 ng/mL
• Méthode de détection: colorimetric
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... CST3(1471)

Description

Description générale

The Human Cystatin C ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human cystatin C in serum, plasma, cell culture supernatants and urine.

Immunogène

Recombinant Human CystatinC

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Actions biochimiques/physiologiques

Cystatin C (CysC) is released by all nucleated cells. Abnormal thyroid function, immunosuppressive therapy and systemic inflammation alter the levels of CysC. Mutation in the gene encoding CysC causes hereditary cystatin C amyloid angiopathy, which is a consequence of amyloid deposits containing the CysC variant in the brain.

Cystatin-C precursor (CST3) is an inhibitor of lysosomal cysteine proteases and several cathepsins like cathepsin S, a lysosomal enzyme. It has been shown that CST3 is a marker of glomerular filtration and diabetic kidney disease. In the presence of pathological conditions, it forms amyloid fibrils in brain arteries. Mutations in the gene encoding CST3 have been shown to be associated with a form of amyloidosis and age-related macular degeneration.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Composants de kit également disponibles séparément

• RABELADE ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Warning
• Mentions de danger: H290
• Conseils de prudence: P234 - P390
• Classification des risques: Met. Corr. 1
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials