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R7876

Sigma-Aldrich

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

Synonyme(s) :

Transfer RNA, tRNA

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About This Item

Numéro CAS:
9014-25-9
Numéro MDL:
MFCD00132200
Code UNSPSC :
12352106
Nomenclature NACRES :
NA.55

Qualité

for molecular biology

Niveau de qualité

300

Type

Type V

Forme

lyophilized powder

Activité spécifique

15-19 units/mg solid

Conditions de stockage

desiccated

Solubilité

H2O: ~2 mg/mL, clear to hazy

Température de stockage

2-8°C

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Description générale

Wheat germ tRNA contains many different specific tRNA molecules.

Application

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Composants

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Définition de l'unité

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening.
Bernard Thisse et al.
Methods in cell biology, 77, 505-519 (2004-12-18)
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Nature protocols, 3(1), 59-69 (2008-01-15)
The in situ hybridization (ISH) technique allows the sites of expression of particular genes to be detected. This protocol describes ISH of digoxigenin-labeled antisense RNA probes to whole-mount zebrafish embryos. In our method, PCR-amplified sequence of a gene of interest
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Nature genetics, 30(3), 329-334 (2002-02-19)
Post-translational modification of histone tails is thought to modulate higher-order chromatin structure. Combinations of modifications including acetylation, phosphorylation and methylation have been proposed to provide marks recognized by specific proteins. This is exemplified, in both mammalian cells and fission yeast
Yana P Blokhina et al.
PLoS genetics, 15(1), e1007730-e1007730 (2019-01-18)
Meiosis is a cellular program that generates haploid gametes for sexual reproduction. While chromosome events that contribute to reducing ploidy (homologous chromosome pairing, synapsis, and recombination) are well conserved, their execution varies across species and even between sexes of the
Crystal E Fagan et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9716-9721 (2013-05-01)
After four decades of research aimed at understanding tRNA selection on the ribosome, the mechanism by which ribosomal ambiguity (ram) mutations promote miscoding remains unclear. Here, we present two X-ray crystal structures of the Thermus thermophilus 70S ribosome containing 16S
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