R1028
Restorase® DNA Polymerase with 10× Reaction Buffer
Enzyme blend for PCR amplification of damaged DNA
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.55
Produits recommandés
Forme
liquid
Utilisation
sufficient for 50 reactions
Caractéristiques
Long & Accurate PCR
dNTPs included: no
hotstart: no
Concentration
2.5 units/μL
Technique(s)
PCR: suitable
Couleur
colorless
Entrée
purified DNA
Conditions d'expédition
wet ice
Température de stockage
−20°C
Description générale
Restorase DNA Polymerase with 10× Reaction Buffer combines Sigma′s long and accurate enzyme technology with a small amount of DNA repair enzyme. The optimized blend will initiate the repair and further amplification of damaged DNA templates greater than 800 bp. Restorase has also been shown to increase yield on undamaged DNA templates.
Application
Restorase® DNA Polymerase with 10× Reaction Buffer has been used in DNA repair.
Actions biochimiques/physiologiques
DNA templates are often damaged on exposure to heat, acids, alkylating agents, and light. These damaged templates result in inefficient or failed DNA amplification by polymerase chain reaction (PCR). Restorase® DNA Polymerase repairs the damaged sites on the DNA templates and initiates subsequent template copying. Restorase treatment of the DNA depends on the level of template damage.
Caractéristiques et avantages
- Reliable amplification of damaged DNA
- When thermostable polymerases fail, this enzyme amplifies the sequence efficiently
- Efficient amplification of sequences in multiplex reactions
- Increased yield and amplicon specificity
- Amplifies a broad spectrum of amplicon sizes varying from 200 bp to 20 kb
- Can repair 3′ bungs, nicks, and abasic sites
Conditionnement
The enzyme is provided with an optimized 10× reaction buffer provided as 1 vial/250 units.
Autres remarques
Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.
View more detailed information on Restorase DNA Polymerase at www.sigma-aldrich.com/restorase.
Informations légales
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Restorase is a registered trademark of Merck KGaA, Darmstadt, Germany
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3
Code de la classe de stockage
10 - Combustible liquids
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Francesca Zinetti et al.
PloS one, 8(6), e65746-e65746 (2013-06-12)
There is increasing evidence that most parapatric cryptic/sister taxa are reproductively compatible across their areas of contact. Consequently, the biological species concept, which assumes absence of interbreeding, is becoming a not so effective criterion in evolutionary ecology. Nevertheless, the few
Mehrdad Hajibabaei et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 360(1462), 1959-1967 (2005-10-11)
Large-scale DNA barcoding projects are now moving toward activation while the creation of a comprehensive barcode library for eukaryotes will ultimately require the acquisition of some 100 million barcodes. To satisfy this need, analytical facilities must adopt protocols that can
Innis, M.A., et al.
PCR Protocols: A Guide to Methods and Applications (1990)
James M Robertson et al.
Forensic science international. Genetics, 12, 168-180 (2014-07-06)
Forensic scientists have used several approaches to obtain short tandem repeat (STR) profiles from compromised DNA samples, including supplementing the polymerase chain reaction (PCR) with enhancers and using procedures yielding reduced-length amplicons. For degraded DNA, the peak intensities of the
Shigeo Yagi et al.
Parasitology research, 102(2), 211-217 (2007-09-28)
Granulomatous amoebic encephalitis (GAE) is a usually fatal disease caused by the free-living amoebae Balamuthia mandrillaris and Acanthamoeba spp. The intent of this study was to determine if the polymerase chain reaction (PCR) could be used retrospectively to detect amoeba
Articles
Restorase® was developed for researchers unable to achieve amplification of damaged DNA templates when using other commercially available DNA polymerases.
Protocoles
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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