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PNS1010

Sigma-Aldrich

Stabilyser Reagent

for stabilization of DNA, RNA, and protein in tissue extracts

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

Forme

liquid

Niveau de qualité

200

Caractéristiques

for stabilization of DNA, RNA, and protein in tissue extracts

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

The stability of protein and antibody structure and function is dependent on a variety of factors such as temperature, freeze/thaw cycles, proteases and pH. For some proteins and antibodies, function can be lost completely with a single freeze/thaw cycle and proteases can significantly degrade proteins and antibodies within hours even when kept at 4 °C. Stabilyser Reagent minimizes the risk to improve protein assay quality and accuracy.

Stabilyser Reagent is a proprietary formulation of detergents and solutes optimized for efficient extraction, stabilization and storage of protein, DNA, and RNA from tissue samples. The unique composition of Stabilyser enables isolation of functionally active proteins, while maintaining integrity of nucleic acids. Stabilization of proteins and RNA is achieved even after 24 hours at room temperature Stabilyser reagent is compatible with Western blotting protocols, functional protein activity assays, and nucleic acid applications like qPCR.

Use in conjunction with a homognenizer for simultaneous tissue lysis and stabilization of protein and nucleic acids in tissue samples.

Application

Prevents degradation of proteins and nucleic acid during tissue lysis and storage. Stabilize functional proteins and RNA.

Caractéristiques et avantages

  • Gentle, non-denaturing lysis buffer does not interfere with downstream applications
  • Convenient ready-to-use reagent
  • Stabilizes RNA and protein for analysis at a later date

Notes préparatoires

Ready-to-use reagent. In general it is recommended to start optimization with 20 μL of Stabilyser reagent per mg of tissue.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Articles

RNA Stabilization and Protein Stabilization in Tissue Extracts with Stabilyser™ Reagent

Stabilize complete, uniform tissue homogenates. Maintain RNA and functional proteins with Stabilyser reagent, the only reagent that protects the integrity of nucleic acid and active protein in one uniform lysate mixture.

Contenu apparenté

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

Lyse et extraction des protéines

Panorama des méthodes de lyse cellulaire et d'extraction protéique, comme la solubilisation par détergents, la lyse par congélation/décongélation, le choc osmotique, la sonication, la lyse cellulaire enzymatique et les techniques de fragmentation mécanique comme l'homogénéisation au Dounce, au Polytron ou au mortier et au pilon.

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

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