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Key Documents

P6993

Sigma-Aldrich

Protein Phosphatase 2A1 bovine

≥1500 units/mg protein

Synonyme(s) :

PPA2A1

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About This Item

Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

bovine

Niveau de qualité

Pureté

≥90% (SDS-PAGE)

Forme

solution

Activité spécifique

≥1500 units/mg protein

Poids mol.

192 kDa

Conditionnement

vial of 1 μg

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

Protein Phosphatase 2A1 (PP2A1) belongs to the PP2A family and comprises trimeric A, B and C subunits. PPA2 enzymes are serine/threonine phosphatases and exist as several isoforms.

Application

Protein phosphatase 2A1 has been used to treat human fibroblast cells prior to western blot analysis.

Actions biochimiques/physiologiques

Protein Phosphatase 2A is a cytoplasmic protein, which colocalizes with microtubule proteins and is involved in the dephosphorylation of the tau protein and oncoprotein 18. Protein Phosphatase 2A1 (PP2A1) binds to polymerized microtubule proteins and may be targeted by tubulin in modulating phosphatase activity. PP2A1 is implicated as a growth suppressor and is associated with dysregulation in cancer. It also regulates cell cycle, RNA splicing differentiation, and signal transduction. PP2A dysfunction is correlated to the tau protein deregulation in Alzheimer′s disease pathophysiology. Protein Phosphatase 2A1 is a divalent cation-dependent protein serine/threonine phosphatase implicated as a growth suppressor and is associated with dysregulation in cancer.

Définition de l'unité

One unit will release 1.0 nanomole of phosphate from 32P-labeled phosphorylase A per minute at pH 7.0 at 30 °C.

Forme physique

Solution in 50 mM Tris-HCl, pH 7.0, containing 14 mM 2-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, and 50% glycerol

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

K Webley et al.
Molecular and cellular biology, 20(8), 2803-2808 (2000-03-25)
Replicative senescence in human fibroblasts is absolutely dependent on the function of the phosphoprotein p53 and correlates with activation of p53-dependent transcription. However, no evidence for posttranslational modification of p53 in senescence has been presented, raising the possibility that changes
A Hiraga et al.
The Biochemical journal, 346 Pt 2, 433-439 (2000-03-24)
Protein phosphatase (PP) 2A1, a trimer composed of A-, B- and C-subunits in the PP2A family, has been regarded as a principal form localizing at microtubules (MT), but PP2A2, the dimer of A- and C-subunits, has not. Substantiating the claim

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