Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

P5813

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone BP53-12, ascites fluid

Synonyme(s) :

Anti-TRP53, Anti-Transformation-related protein 53

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

BP53-12, monoclonal

Poids mol.

antigen 53 kDa

Contient

15 mM sodium azide

Espèces réactives

human

Technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using methacarn-fixed, paraffin-embedded sections of human malignant tissue
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: suitable using cell lysates from human cell lines known to express high levels of the p53 protein

Isotype

IgG2a

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TP53(7157)

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Monoclonal Anti-p53 (mouse Ig2a isotype) is derived from the BP53-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human wild-type p53 protein. The tumor suppressor gene TP53 (gene) codes for a transcription factor. TP53 gene is mapped to human chromosome 17p13.1. The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells.

Immunogène

recombinant human wild-type p53 protein.

Application

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • western blotting
  • immunohistochemistry
  • immunocytochemistry
  • enzyme linked immunosorbent assay (ELISA)

Mouse monoclonal clone BP53-12 anti-p53 antibody is a useful tool suitable for the detection and quantitation of p53 applying various immunochemical techniques, especially immunohistochemistry using conventionally fixed and processed histological sections. The product is also useful for immunoprecipitation, ELISA and immunocytochemical staining of cultured cell lines.

Actions biochimiques/physiologiques

The tumor suppressor gene TP53 (gene) gene is involved in the regulation of cell cycle, senescence and apoptosis upon oncogene activation, stress and DNA damage. Point mutations of the wild-type gene have been suggested to be a key event in the development of malignancy as the mutant protein acts as a dominant oncogene. Indeed, mutations of the p53 gene are the most common molecular changes identified in human cancer. They have been reported to be a frequent feature of breast, lung, colon, ovarian, brain, testicular and bladder cancers, melanoma, neurofibrosarcoma, and certain types of leukemia. The accumulation of high levels of p53 is a potential novel marker for malignancy. Monoclonal Anti-p53 recognizes a denaturation resistant epitope on the primate p53 nuclear protein (53 kDa). It does not react with other cellular proteins.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Dominant-negative p53 mutant R248Q increases the motile and invasive activities of oral squamous cell carcinoma cells
Nakazawa S, et al.
Biomedical Research, 40(1), 37-49 (2019)
How narrow-band and broad-band uvb irradiation influences the histomorphology evaluations of experimental animals? skin-a comparative study
Borowska K
Dermatology Online Journal, 8(3e), e5-e5 (2017)
J Bártková et al.
International journal of cancer, 49(2), 196-202 (1991-09-09)
Expression of the p53 oncoprotein was examined in a wide range of primary human testicular germ-cell tumours using a new mouse monoclonal antibody (MAb) BP53-11 raised and characterized in this study, in parallel with a polyclonal rabbit antiserum CM-1. Immunohistochemistry
A p53-like protein from a freshwater mollusc Lamellidens corrianus
Mohanty BP
Indian Journal of Biochemistry & Biophysics, 121-139 (2006)
Identification and characterization of different subpopulations in a human lung adenocarcinoma cell line (A549)
Croce MV, et al.
Pathology Oncology Research, 5(3), 197-204 (1999)

Articles

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique