P3818

Protein Disulfide Isomerase from bovine liver

≥100 units/mg protein, lyophilized powder

• Synonyme(s) : PDI
• Numéro CAS: 37318-49-3
• Numéro de classification (Commission des enzymes): 5.3.4.1 (BRENDA, IUBMB)
• Numéro MDL: MFCD00163364
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 200
• Activité spécifique: ≥100 units/mg protein
• Poids mol.: 107 kDa
• Composition: Protein, ~10% Lowry
• Température de stockage: −20°C
• InChI: 1S/C7H5Cl2NO5S/c8-4-2-5(9)6(16(13,14)15-10)1-3(4)7(11)12/h1-2H,10H2,(H,11,12)
• Clé InChI: DHUYKLYJBKXDBM-UHFFFAOYSA-N

Description

Description générale

Protein disulfide isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa with the monomer corresponding to 57 kDa. The isoelectric point (pI) is approximately 4.2. The enzyme is a glycoprotein with 12% total carbohydrate content comprising of mannose, galactose, N-acetyl neuraminic acid (NANA) and 2-acetamido-2-deoxyglucose. PDI is an ubiquitous redox chaperone enzyme. It belongs to the, thioredoxin superfamily and is high conserved.

Application

Protein Disulfide Isomerase from bovine liver has been used:

• to study the functional role of PDI in parasite infection and the interaction between macrophage PDI and L. chagasi
• in the in vitro translation reaction for the generation of disulfide bonds
• in insulin-disulfide reduction assay and peptide binding assay
• as a positive control in thiol-disulfide oxidoreductase activity assay

Actions biochimiques/physiologiques

Protein Disulfide Isomerase (PDI) is mainly located in the endoplasmic reticulum (ER), where it assists in protein-folding and thiol-disulfide exchanges. It aids protein refolding in vitro allowing recombinant proteins to achieve their native state.

Protein Disulfide Isomerase(PDI) has the C-terminal ER retention sequence Lys-Asp-Glu-Leu. It has active, intracellular traffic to different cell compartments. PDI supports internalization of Chlamydia, cholera and diphtheria toxins in some hosts. PDI is required for Sindbis virus infection and aids in reducing HIV gp120 protein thiols. PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings.

Conditionnement

Package size based on protein content.

Propriétés physiques

Protein Disulfide Isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa (gel filtration) and the molecular weight of the monomer has been reported at 57 kDA (SDS-PAGE). The enzyme is a glycoprotein with 12% total carbohydrate content, composed of 4.6% mannose, 2.5% galactose, 1.4% NANA, and 3.5% 2-acetamido-2-deoxyglucose.

Définition de l'unité

One unit cause a change in A₆₅₀ of 0.01 per min of a 1.0 mg/mL solution of insulin in the presence of dithiothreitol at pH 7.5 at 25 °C.

Forme physique

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)