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P3164

Sigma-Aldrich

Anti-Pig Serum antibody produced in rabbit

whole antiserum

Synonyme(s) :

Anti-Serum, pig

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About This Item

Numéro MDL:
MFCD00162754
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

whole antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Contient

15 mM sodium azide

Espèces réactives

pig

Technique(s)

immunoelectrophoresis: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Guinea pig serum is highly enriched with L-asparaginase activity. This L-asparaginase activity imparts inhibitory or anti-lymphoma effects to pig serum.

Spécificité

Strong reactivity with normal pig serum has been determined.

Application

Anti-Pig Serum antibody produced in rabbit has been used in differential staining.
Anti-Pig Serum antibody produced in rabbit has been used in the dual differential staining of blastocysts to assess the number of inner cell mass (ICM) cells and trophectoderm (TE) cells.

Qualité

The antiserum has been treated to remove lipoproteins.

Forme physique

Rabbit Anti-Pig Serum is provided as a liquid containing 0.1% sodium azide as preservative.

Stockage et stabilité

For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Koji Yoshioka et al.
Biology of reproduction, 66(1), 112-119 (2001-12-26)
We evaluated the in vitro development of porcine zygotes that were cultured in a novel culture medium, porcine zygote medium (PZM), under different conditions and compared to in vivo development. The viability of these zygotes to full term after culture
Z Macháty et al.
Biology of reproduction, 59(2), 451-455 (1998-08-01)
In vitro development of early porcine embryos under different culture conditions was evaluated and compared to in vivo development. First, one- and two-cell embryos were collected and cultured individually in 20- microl drops under 5% CO2 in air for 4
Evidence that the L-Asparaginase Activity of Guinea Pig Serum is responsible for its Antilymphoma Effects
Broome JD
Nature, 191, 1114-1115 (1961)
Z Macháty et al.
Biology of reproduction, 57(5), 1123-1127 (1997-11-22)
Thimerosal (200 microM) triggered Ca2+ oscillations in 56 of 56 mature porcine oocytes. The Ca2+ oscillations were blocked by the sulfhydryl-reducing agent dithiothreitol (DTT), thus supporting the hypothesis that thimerosal acts by oxidizing critical sulfhydryl groups on intracellular Ca2+-release proteins.
Tao Lin et al.
Reproductive toxicology (Elmsford, N.Y.), 75, 96-109 (2017-12-17)
In this study, we used a pig model to investigate the effects of α-solanine (a natural toxin found mainly in potato sprouts) on oocyte maturation, quality and subsequent embryonic development. We found that α-solanine (10 μM) disturbed meiotic resumption and increased
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