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Key Documents

P2859

Sigma-Aldrich

Anti-p300/CBP antibody, Mouse monoclonal

clone NM11, purified from hybridoma cell culture

Synonyme(s) :

Anti-CREB Binding Protein

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

NM11, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen 300 kDa

Espèces réactives

mouse, rat, human, mink, monkey

Concentration

~2 mg/mL

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 10-20 μg/mL using human 293 embryonal kidney cells

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... KAT2B(8850)
mouse ... Kat2b(18519)
rat ... Pcaf(301164)

Description générale

Monoclonal Anti-p300/CBP (mouse IgG1 isotype) is derived from the NM11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a native human p300. The KAT2B (lysine acetyltransferase 2B) gene encodes a p300/CBP (CREB binding protein)-associated factor, which belongs to the family of GNAT (GCN5 (general control nonderepressible 5)-related N-acetyltransferase).

Spécificité

Reacts specifically with both p300 and CBP but not with the related p270 molecule. The epitope recognized by the antibody resides within the 21 amino acid stretch spanning amino acids 2071-2091 near the CBP C-terminus. CBP and p300 differ by three noncontiguous residues within this 21 amino acid region, a difference that does not detectably affect the reactivity of the antibody.

Immunogène

native human p300.

Actions biochimiques/physiologiques

p300/CBP are capable of binding to a variety of transcriptional activator and regulatory molecules, including p53 and nuclear hormone receptors. The complexity of these p300/CBP cellular associations suggests that both proteins play a central role in the coordination of gene expression during cell growth and differentiation.
KAT2B (lysine acetyltransferase 2B) possess histone acetyltransferase (HAT) activity and is thus known to regulate transcription process. Acetylation also influences the chromatin structure. Lysine acetylation significantly contributes to protein modification. It is essential for establishing protein function by altering its structure, activity and molecular interaction.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

E1A promotes association between p300 and pRB in multimeric complexes required for normal biological activity.
Wang H G, et al.
Journal of Virology, 69(12), 7917-7924 (1995)
Characterization of monoclonal antibodies raised against p300: both p300 and CBP are present in intracellular TBP complexes.
Dallas P B, et al.
Journal of Virology, 71(2), 1726-1731 (1997)
Adenoviral E1A-associated protein p300 as a functional homologue of the transcriptional co-activator CBP.
Lundblad j R, et al.
Nature, 74(6517), 85-85 (1995)
Differential Effects of Histone Acetyltransferase GCN5 or PCAF Knockdown on Urothelial Carcinoma Cells.
Koutsogiannouli E A, et al.
International Journal of Molecular Sciences, 18(7), 1449-1449 (2017)
Monoclonal Antibody NM11 Recognizes a C-Terminal Epitope Shared by p300 and CBP
Dallas P B, et al.
Hybridoma, 16(3), 273-275 (1997)

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