P2714
Protease Inhibitor Cocktail
lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder
Synonyme(s) :
Protease Inhibitor Cocktail from bovine lung, Protease Inhibitor Mix, Protease inhibitor
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77
Produits recommandés
product name
Protease Inhibitor Cocktail powder, for general use, lyophilized powder
Source biologique
bovine lung
Niveau de qualité
Forme
lyophilized powder
Température de stockage
−20°C
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Description générale
The protease inhibitor cocktail has been optimized and tested for general use. It consists of water-soluble protease inhibitors that effectively inhibit serine, cysteine, and metalloproteases. This product is supplied as a lyophilized powder.
Spécificité
Inhibits serine, cysteine, and metallo-proteases.
Application
Protease Inhibitor Cocktail powder has been used as a component:
- in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis
- in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)
- in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification
- in 0.1 M NaCl solution to wash and collect skin secretions of frog
Actions biochimiques/physiologiques
The components of this mixture, such as AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
Composants
This mixture contains individual components, including:
AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM
Each component has specific inhibitory properties.
AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM
Each component has specific inhibitory properties.
Attention
The lyophilized powder is stable for 4 years when stored at -20°C.
Quantité
One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Notes préparatoires
This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.
Autres remarques
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Articles
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
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