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P1867

Plasmin from human plasma

Name: Plasmin from human plasma
Brand: SIGMA

lyophilized powder, ≥2.0 units/mg protein

Synonyme(s) :

Fibrinolysin

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About This Item

Numéro CAS:

9001-90-5

Numéro de classification (Commission des enzymes):

3.4.21.7 (BRENDA, IUBMB)

Numéro CE :

232-640-3

Numéro MDL:

MFCD00131921

Code UNSPSC :

12352204

eCl@ss :

42030239

Nomenclature NACRES :

NA.54

Forme

lyophilized powder

Niveau de qualité

200

Activité spécifique

≥2.0 units/mg protein

Impuretés

HIV, Hepatitis B and Hepatitis C, tested negative

Numéro d'accès UniProt

P00747

Application(s)

diagnostic assay manufacturing

Température de stockage

−20°C

Informations sur le gène

human ... PLG(5340)

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Description générale

Plasmin functions as a key enzyme of the fibrinolytic cascade, and is also important in inflammation processes.

Application

A complex between plasmin and an inhibitor has been isolated in a study via affinity chromatography from urokinase-activated human plasma. It has also been used in a study to investigate activation of human epithelial sodium channel (ENaC) by plasmin and chymotrypsin.

Actions biochimiques/physiologiques

It is inhibited by α 2-antiplasmin and its interaction with fibrin is blocked. It digests the N-terminal region of the cytoplasmic protein αsynuclein preventing its uptake by surrounding cells.

Plasmin exhibits preferential cleavage at the carboxyl side of lysine and arginine residues with higher selectivity than trypsin. Converts polymerized fibrin into soluble products.
pH Optimum: 8.5
pH 7.5: ~40% of maximal activity, pH 9.5: ~50% of maximal activity
Temperature Optimum: 37 °C with rapid inactivation at 56 °C

Conditionnement

Package size based on protein content

Définition de l'unité

One unit will produce one μmole of p-Nitroanilide from D-Val-Leu-Lys-p-Nitroanilide per minute at pH 7.5 at 37 °C.

Forme physique

Lyophilized powder containing sodium phosphate, mannitol, and NaCl.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Sigma-Aldrich

A8849

α₂-Antiplasmin from human plasma

Plasmin and chymotrypsin have distinct preferences for channel activating cleavage sites in the γ subunit of the human epithelial sodium channel.

Silke Haerteis et al.

The Journal of general physiology, 140(4), 375-389 (2012-09-12)

Proteolytic activation of the epithelial sodium channel (ENaC) involves cleavage of its γ subunit in a critical region targeted by several proteases. Our aim was to identify cleavage sites in this region that are functionally important for activation of human

Plasmin as a proinflammatory cell activator.

Tatiana Syrovets et al.

Journal of leukocyte biology, 92(3), 509-519 (2012-05-09)

The serine protease plasmin generated from its zymogen plasminogen is best known for its function as a key enzyme of the fibrinolytic cascade. However, beyond fibrinolysis, plasmin has a number of crucial functions in a variety of processes, including inflammation.

The primary inhibitor of plasmin in human plasma.

S Müllertz et al.

The Biochemical journal, 159(3), 545-553 (1976-12-01)

A complex between plasmin and an inhibitor was isolated by affinity chromatography from urokinase-activated human plasma. The complex did not react with antibodies against any of the known proteinase inhibitors in plasma. A rabbit antiserum against the complex was produced.

On the mechanism of the reaction between human alpha 2-antiplasmin and plasmin.

B Wiman et al.

The Journal of biological chemistry, 254(18), 9291-9297 (1979-09-25)

A fibrinogen concentrate Haemocomplettan (Riastap) or a Factor XIII concentrate Fibrogammin combined with a mini dose of tranexamic acid can reverse the fibrin instability to fibrinolysis induced by thrombin- or FXa-inhibitor.

Shu He et al.

British journal of haematology, 160(6), 806-816 (2013-01-31)

To assess whether Haemocomplettan(®) (fibrinogen concentrate) or Fibrogammin(®) (Factor XIII concentrate) can be used to manage bleeding complications of antithrombotic treatment, we examined a normal plasma pool spiked with AR-H067637 (thrombin inhibitor) or rivaroxaban (activated factor X-inhibitor), to which one

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Protocoles

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Protocol for Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride