P0091
PolyFreeze
water-soluble support matrix
Synonyme(s) :
water-soluble support matrix for frozen sectioning
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About This Item
Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47
Produits recommandés
Niveau de qualité
Couleur
colorless
Densité
1.030 g/cm3
Application(s)
hematology
histology
Température de stockage
room temp
Description générale
PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C. Snap freeze using PolyFreeze with isopentane and liquid nitrogen, or dry ice (slush/slurry or bunker). Store specimens frozen in PolyFreeze in liquid nitrogen canisters or in airtight containers in a -80 °C freezer. PolyFreeze does not cause autofluorescence and can easily be washed away during fixation and rinsed prior to staining. Once rinsed, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions.
Application
PolyFreeze has been used as an embedding medium for frozen sectioning of plant and animal samples.
Notes préparatoires
PolyFreeze is supplied ready-to-use. A cloudy appearance of the unfrozen product has no effect on its performance.
Stockage et stabilité
Store the product at room temperature
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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