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NA1020

Sigma-Aldrich

GenElute™ PCR Clean-Up Kit

sufficient for 70 purifications

Synonyme(s) :

Gen Elute, PCR Purification

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.52

Utilisation

sufficient for 70 purifications

Niveau de qualité

200

Technique(s)

DNA purification: suitable

Température de stockage

15-25°C

Description générale

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Application

GenElute™ PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Caractéristiques et avantages

  • Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
  • Recovers up to 95% of PCR products between 100 bp and 10 kb
  • Removes over 99% of primers and other components
  • Eliminates the need to remove mineral oil by organic extraction
  • 40% more purification preps supplied than market leader

Autres remarques

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Principe

The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed).

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Pictogrammes

CorrosionExclamation mark
GHS05,GHS07

Mention d'avertissement

Warning

Mentions de danger

H290,H302,H315,H319,H336

Classification des risques

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Central nervous system

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

GenElute™ PCR Clean-Up Procedure

Follow this procedure to rapidly purify single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from excess primers, nucleotides, DNA polymerase, oil and salts using the GenElute™ PCR Clean-up Kit.

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